FOG02487
EOG87PVP7

sce:PRP16

Genes: 24

Protein description
RNA degradation


SGD Description
DEAH-box RNA helicase involved in second catalytic step of splicing and in exon ligation; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step; missense mutation in human ortholog DHX38 associated with early-onset retinitis pigmentosa


PomBase Description
ATP-dependent RNA helicase Prp16


AspGD Description
Ortholog(s) have U2-type spliceosomal complex localization


References

Burgess S, et al. (1990 Mar 9). A putative ATP binding protein influences the fidelity of branchpoint recognition in yeast splicing.

Schwer B, et al. (1991 Feb 7). PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 1 genes with posterior transmembrane prediction > 50%

FOG02488
EOG87PVP7

sce:PRP22

Genes: 24

Protein description
RNA degradation


SGD Description
DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction


PomBase Description
ATP-dependent RNA helicase Prp22


AspGD Description
Ortholog(s) have U2-type spliceosomal complex localization


References

Company M, et al. (1991 Feb 7). Requirement of the RNA helicase-like protein PRP22 for release of messenger RNA from spliceosomes.

Ohi MD, et al. (2002 Apr). Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs.

Carnahan RH, et al. (2005 Mar). Dim1p is required for efficient splicing and export of mRNA encoding lid1p, a component of the fission yeast anaphase-promoting complex.

Beltrao P, et al. (2009 Jun 16). Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Ren L, et al. (2011 Feb 28). Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.

Arita Y, et al. (2011 May). Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.

Livesay SB, et al. (2013 Nov). Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.

Bayne EH, et al. (2014). A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.

Kallgren SP, et al. (2014). The proper splicing of RNAi factors is critical for pericentric heterochromatin assembly in fission yeast.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Chen W, et al. (2014 Mar). Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.

Lipp JJ, et al. (2015 Aug). SR protein kinases promote splicing of nonconsensus introns.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG02489
EOG87PVP7

sce:PRP43

Genes: 32

Protein description
RNA degradation


SGD Description
RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p


PomBase Description
ATP-dependent RNA helicase Prp43


AspGD Description
Ortholog(s) have ATP-dependent RNA helicase activity, mRNA binding activity


References

Arenas JE, et al. (1997 Oct 28). Prp43: An RNA helicase-like factor involved in spliceosome disassembly.

Tsai RT, et al. (2005 Dec 15). Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2.

Gruhler A, et al. (2005 Mar). Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.

Boon KL, et al. (2006 Aug). Yeast ntr1/spp382 mediates prp43 function in postspliceosomes.

Chi A, et al. (2007 Feb 13). Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.

Ren L, et al. (2011 Feb 28). Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.

Snaith HA, et al. (2011 Jul 1). Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.

Nie M, et al. (2012 Aug 24). Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.

Grenier St-Sauveur V, et al. (2013 Dec). Poly(A) tail-mediated gene regulation by opposing roles of Nab2 and Pab2 nuclear poly(A)-binding proteins in pre-mRNA decay.

Livesay SB, et al. (2013 Nov). Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.

Lee NN, et al. (2013 Nov 21). Mtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance.

Wang J, et al. (2013 Sep 1). Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Kallgren SP, et al. (2014). The proper splicing of RNAi factors is critical for pericentric heterochromatin assembly in fission yeast.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Chen W, et al. (2014 Mar). Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 4 genes with posterior transmembrane prediction > 50%

FOG02490
EOG87PVP7

sce:PRP2

Genes: 22

Protein description
RNA degradation


SGD Description
RNA-dependent DExD/H-box ATPase; required for activation of spliceosome before first transesterification step in RNA splicing; implicated in rearranging and proofreading snRNA structure in catalytic activation of spliceosome; ortholog of human protein DHX16


PomBase Description
ATP-dependent RNA helicase Cdc28


AspGD Description
Has domain(s) with predicted ATP binding, ATP-dependent helicase activity, helicase activity, nucleic acid binding activity


References

Nasmyth K, et al. (1981). Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe.

Last RL, et al. (1986 Dec). Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins.

Hirano T, et al. (1986 Nov). Isolation and characterization of Schizosaccharomyces pombe cutmutants that block nuclear division but not cytokinesis.

Chen JH, et al. (1990 Nov 11). The yeast PRP2 protein, a putative RNA-dependent ATPase, shares extensive sequence homology with two other pre-mRNA splicing factors.

King DS, et al. (1990 Nov 25). Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae.

Kim SH, et al. (1992 Jun). The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase.

Plumpton M, et al. (1994 Feb 15). A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes.

Teigelkamp S, et al. (1994 Feb 15). The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNA.

Roy J, et al. (1995 Jun). The final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing.

Kim SH, et al. (1996 Dec). Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing.

Lundgren K, et al. (1996 Jul). A connection between pre-mRNA splicing and the cell cycle in fission yeast: cdc28+ is allelic with prp8+ and encodes an RNA-dependent ATPase/helicase.

Urushiyama S, et al. (1996 Nov 27). Isolation of novel pre-mRNA splicing mutants of Schizosaccharomyces pombe.

Habara Y, et al. (2001 May). Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe.

Silverman EJ, et al. (2004 Dec). Interaction between a G-patch protein and a spliceosomal DEXD/H-box ATPase that is critical for splicing.

Wilkinson CR, et al. (2004 Dec 29). Ubiquitin-like protein Hub1 is required for pre-mRNA splicing and localization of an essential splicing factor in fission yeast.

Edwalds-Gilbert G, et al. (2004 Feb). Definition of a spliceosome interaction domain in yeast Prp2 ATPase.

Shimada M, et al. (2005 Sep 23). Regulation of Cdc2p and Cdc13p is required for cell cycle arrest induced by defective RNA splicing in fission yeast.

Chinen M, et al. (2010 Feb 19). Involvement of the spliceosomal U4 small nuclear RNA in heterochromatic gene silencing at fission yeast centromeres.

Ren L, et al. (2011 Feb 28). Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.

Watanabe N, et al. (2012 Feb 3). Characterization of the ptr5+ gene involved in nuclear mRNA export in fission yeast.

Van Damme P, et al. (2012 Jul 31). N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

Livesay SB, et al. (2013 Nov). Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Mayerle M, et al. (2016 May). Prp8 retinitis pigmentosa mutants cause defects in the transition between the catalytic steps of splicing.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 13 genes with posterior transmembrane prediction > 50%

FOG02491
EOG87PVP7

sce:DHR2

Genes: 25

Protein description
RNA degradation


SGD Description
Predominantly nucleolar DEAH-box ATP-dependent RNA helicase; required for 18S rRNA synthesis


PomBase Description
ATP-dependent RNA helicase Prh1 (predicted)


AspGD Description
Has domain(s) with predicted ATP binding, ATP-dependent helicase activity, helicase activity, nucleic acid binding activity


References

Colley A, et al. (2000 Oct). Dhr1p, a putative DEAH-box RNA helicase, is associated with the box C+D snoRNP U3.

Choque E, et al. (2011 Nov-Dec). The nucleolar protein Nop19p interacts preferentially with Utp25p and Dhr2p and is essential for the production of the 40S ribosomal subunit in Saccharomyces cerevisiae.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 1 genes with posterior transmembrane prediction > 50%

FOG02492


sce:absent

Genes: 0

 





 

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG02493
EOG87PVP7

sce:ECM16

Genes: 33

SGD Description
Essential DEAH-box ATP-dependent RNA helicase specific to U3 snoRNP; predominantly nucleolar in distribution; required for 18S rRNA synthesis


PomBase Description
ATP-dependent RNA helicase Dhr1 (predicted)


AspGD Description
Ortholog(s) have RNA helicase activity, role in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) and 90S preribosome, mitochondrion, nucleolus, small-subunit processome localization


References

Colley A, et al. (2000 Oct). Dhr1p, a putative DEAH-box RNA helicase, is associated with the box C+D snoRNP U3.

Dragon F, et al. (2002 Jun 27). A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis.

Wang J, et al. (2013 Sep 1). Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 5 genes with posterior transmembrane prediction > 50%

FOG02494
EOG87PVP7

sce:absent

Genes: 7

PomBase Description
ATP-dependent RNA helicase, spliceosomal (predicted)


AspGD Description
Has domain(s) with predicted ATP binding, ATP-dependent helicase activity, helicase activity, nucleic acid binding activity


References

Kennedy PJ, et al. (2008 Nov). A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.

Ren L, et al. (2011 Feb 28). Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.

Pan X, et al. (2012 Nov 23). Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.

Kallgren SP, et al. (2014). The proper splicing of RNAi factors is critical for pericentric heterochromatin assembly in fission yeast.

Anver S, et al. (2014 Aug). Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.

Sideri T, et al. (2014 Dec 1). Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.

Zhou H, et al. (2015 Oct). Genome-wide screen of fission yeast mutants for sensitivity to 6-azauracil, an inhibitor of transcriptional elongation.

Dudin O, et al. (2017 Apr). A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%