FOG02495
EOG8H44JM

sce:DNF2;DNF1

Genes: 56

SGD Description
Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF2 has a paralog, DNF1, that arose from the whole genome duplication|Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF1 has a paralog, DNF2, that arose from the whole genome duplication


PomBase Description
P-type ATPase (predicted)


AspGD Description
Has domain(s) with predicted ATP binding, cation-transporting ATPase activity, magnesium ion binding, metal ion binding, nucleotide binding, phospholipid-translocating ATPase activity and role in cation transport, phospholipid transport|Ortholog(s) have ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism, phospholipid-translocating ATPase activity


References

Hua Z, et al. (2002 Sep). An essential subfamily of Drs2p-related P-type ATPases is required for protein trafficking between Golgi complex and endosomal/vacuolar system.

Gruhler A, et al. (2005 Mar). Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.

Kim H, et al. (2006 Jul 25). A global topology map of the Saccharomyces cerevisiae membrane proteome.

Chi A, et al. (2007 Feb 13). Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.

Nakano K, et al. (2008 Apr). Protein kinases Fpk1p and Fpk2p are novel regulators of phospholipid asymmetry.

Wendland J, et al. (2011 Dec). Genome evolution in the eremothecium clade of the Saccharomyces complex revealed by comparative genomics.

Starita LM, et al. (2012 Jan). Sites of ubiquitin attachment in Saccharomyces cerevisiae.

Schultzhaus Z, et al. (2015 Jul). Aspergillus nidulans flippase DnfA is cargo of the endocytic collar and plays complementary roles in growth and phosphatidylserine asymmetry with another flippase, DnfB.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 55 genes with posterior transmembrane prediction > 50%

FOG02496
EOG8H44JM

sce:DRS2

Genes: 34

Protein description
ps transporter


SGD Description
Trans-golgi network aminophospholipid translocase (flippase); maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation, endocytosis, protein trafficking between the Golgi and endosomal system and the cellular response to mating pheromone; autoinhibited by its C-terminal tail; localizes to the trans-Golgi network; mutations in human homolog ATP8B1 result in liver disease


PomBase Description
P-type ATPase (predicted)


AspGD Description
Potassium- or calcium-transporting ATPase


References

Ripmaster TL, et al. (1993 Dec). DRS1 to DRS7, novel genes required for ribosome assembly and function in Saccharomyces cerevisiae.

Gall WE, et al. (2002 Sep 17). Drs2p-dependent formation of exocytic clathrin-coated vesicles in vivo.

Okorokova-Façanha AL, et al. (2003 Jul). An inventory of the P-type ATPases in the fission yeast Schizosaccharomyces pombe.

Saito K, et al. (2004 Jul). Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae.

Kim H, et al. (2006 Jul 25). A global topology map of the Saccharomyces cerevisiae membrane proteome.

Beltrao P, et al. (2009 Jun 16). Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Wendland J, et al. (2011 Dec). Genome evolution in the eremothecium clade of the Saccharomyces complex revealed by comparative genomics.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 34 genes with posterior transmembrane prediction > 50%

FOG02497
EOG8H44JM

sce:NEO1

Genes: 35

SGD Description
Putative aminophospholipid translocase (flippase); involved in endocytosis, vacuolar biogenesis and Golgi to ER vesicle-mediated transport; localizes to endosomes and the Golgi apparatus


PomBase Description
P-type ATPase, calcium transporting (predicted)


AspGD Description
Ortholog(s) have role in endocytosis, retrograde vesicle-mediated transport, Golgi to ER, vacuole organization and COPI-coated vesicle, Golgi membrane, endosome localization


References

Okorokova-Façanha AL, et al. (2003 Jul). An inventory of the P-type ATPases in the fission yeast Schizosaccharomyces pombe.

Wicky S, et al. (2004 Sep). Molecular interactions of yeast Neo1p, an essential member of the Drs2 family of aminophospholipid translocases, and its role in membrane trafficking within the endomembrane system.

Kim H, et al. (2006 Jul 25). A global topology map of the Saccharomyces cerevisiae membrane proteome.

Harris SD, et al. (2009 Mar). Morphology and development in Aspergillus nidulans: a complex puzzle.

Ucisik-Akkaya E, et al. (2014 Apr 11). A genome-wide screen for sporulation-defective mutants in Schizosaccharomyces pombe.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Schultzhaus Z, et al. (2015 Jul). Aspergillus nidulans flippase DnfA is cargo of the endocytic collar and plays complementary roles in growth and phosphatidylserine asymmetry with another flippase, DnfB.

Dudin O, et al. (2017 Apr). A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 35 genes with posterior transmembrane prediction > 50%

FOG02498
EOG8H44JM

sce:DNF3

Genes: 34

SGD Description
Trans-golgi network aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to the maintenance of membrane lipid asymmetry in post-Golgi secretory vesicles; role in protein trafficking between the Golgi and endosomal system; localizes to the trans-Golgi network; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone


PomBase Description
Trans-golgi network aminophospholipid translocase (flippase), Dnf1 (predicted)


AspGD Description
Ca2+/phospholipid-transporting ATPase


References

Kim H, et al. (2006 Jul 25). A global topology map of the Saccharomyces cerevisiae membrane proteome.

Furuta N, et al. (2007 Jan). Endocytic recycling in yeast is regulated by putative phospholipid translocases and the Ypt31p/32p-Rcy1p pathway.

Lenoir G, et al. (2009 Jul 3). Cdc50p plays a vital role in the ATPase reaction cycle of the putative aminophospholipid transporter Drs2p.

Wendland J, et al. (2011 Dec). Genome evolution in the eremothecium clade of the Saccharomyces complex revealed by comparative genomics.

Schultzhaus Z, et al. (2015 Jul). Aspergillus nidulans flippase DnfA is cargo of the endocytic collar and plays complementary roles in growth and phosphatidylserine asymmetry with another flippase, DnfB.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 34 genes with posterior transmembrane prediction > 50%