FOG00259
EOG81896F

sce:RPB7

Genes: 33

Protein description
RNA polymerase subunit


SGD Description
RNA polymerase II subunit B16; forms dissociable heterodimer with Rpb4p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation


PomBase Description
DNA-directed RNA polymerase complex II subunit Rpb7


AspGD Description
DNA-directed RNA polymerase


References

McKune K, et al. (1993 Mar). RPB7, one of two dissociable subunits of yeast RNA polymerase II, is essential for cell viability.

Kimura M, et al. (1997 Oct 10). RNA polymerase II subunits 2, 3, and 11 form a core subassembly with DNA binding activity.

Sakurai H, et al. (1997 Sep 1). Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II.

Ishiguro A, et al. (1998 Jun 19). Two large subunits of the fission yeast RNA polymerase II provide platforms for the assembly of small subunits.

Sakurai H, et al. (1999 Nov). The Rpb4 subunit of fission yeast Schizosaccharomyces pombe RNA polymerase II is essential for cell viability and similar in structure to the corresponding subunits of higher eukaryotes.

Kimura M, et al. (2000 Feb 15). Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II.

Spåhr H, et al. (2000 Jan 14). Purification and characterization of RNA polymerase II holoenzyme from Schizosaccharomyces pombe.

Kimura M, et al. (2001 Feb). Intracellular contents and assembly states of all 12 subunits of the RNA polymerase II in the fission yeast Schizosaccharomyces pombe.

Orlicky SM, et al. (2001 Mar 30). Dissociable Rpb4-Rpb7 subassembly of rna polymerase II binds to single-strand nucleic acid and mediates a post-recruitment step in transcription initiation.

Kimura M, et al. (2002 Mar). Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II.

Sakurai H, et al. (2002 Mar). Level of the RNA polymerase II in the fission yeast stays constant but phosphorylation of its carboxyl terminal domain varies depending on the phase and rate of cell growth.

Mitsuzawa H, et al. (2003 Aug 15). Rpb7 subunit of RNA polymerase II interacts with an RNA-binding protein involved in processing of transcripts.

Kettenberger H, et al. (2003 Aug 8). Architecture of the RNA polymerase II-TFIIS complex and implications for mRNA cleavage.

Armache KJ, et al. (2003 Jun 10). Architecture of initiation-competent 12-subunit RNA polymerase II.

Chung WH, et al. (2003 Oct). RNA polymerase II/TFIIF structure and conserved organization of the initiation complex.

Kamenski T, et al. (2004 Aug 13). Structure and mechanism of RNA polymerase II CTD phosphatases.

Kettenberger H, et al. (2004 Dec 22). Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS.

Tamayo E, et al. (2004 Jun). Mediator is required for activated transcription in a Schizosaccharomyces pombe in vitro system.

Galagan JE, et al. (2005 Dec 22). Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae.

Armache KJ, et al. (2005 Feb 25). Structures of complete RNA polymerase II and its subcomplex, Rpb4/7.

Mitsuzawa H, et al. (2005 Jan 3). Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit.

Hayashi K, et al. (2005 Mar). Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation.

Djupedal I, et al. (2005 Oct 1). RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing.

Selitrennik M, et al. (2006 Dec). Nucleocytoplasmic shuttling of the Rpb4p and Rpb7p subunits of Saccharomyces cerevisiae RNA polymerase II by two pathways.

Kettenberger H, et al. (2006 Jan). Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs.

Meyer PA, et al. (2006 Jun). Phasing RNA polymerase II using intrinsically bound Zn atoms: an updated structural model.

Lotan R, et al. (2007 Sep 24). The Rpb7p subunit of yeast RNA polymerase II plays roles in the two major cytoplasmic mRNA decay mechanisms.

Spåhr H, et al. (2009 Jun 9). Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.

Bah A, et al. (2012 Apr). The telomeric transcriptome of Schizosaccharomyces pombe.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Kriegenburg F, et al. (2014 Jan). A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability.

Suzuki S, et al. (2014 May). A novel method for purification of the endogenously expressed fission yeast Set2 complex.

Kumar D, et al. (2015 Jan). Modulating the level of the Rpb7 subunit of RNA polymerase II affects cell separation in Schizosaccharomyces pombe.

Tucker JF, et al. (2016 Feb). A Novel Epigenetic Silencing Pathway Involving the Highly Conserved 5'-3' Exoribonuclease Dhp1/Rat1/Xrn2 in Schizosaccharomyces pombe.

Suzuki S, et al. (2016 May 19). Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00260
EOG81896F

sce:RPC25

Genes: 33

Protein description
RNA polymerase subunit


SGD Description
RNA polymerase III subunit C25; required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p


PomBase Description
DNA-directed RNA polymerase III complex subunit Rpc25


AspGD Description
Ortholog(s) have RNA polymerase III activity and role in tRNA transcription from RNA polymerase III promoter, transcription initiation from RNA polymerase III type 2 promoter


References

Sadhale PP, et al. (1994 Sep). C25, an essential RNA polymerase III subunit related to the RNA polymerase II subunit RPB7.

Chédin S, et al. (1998). The yeast RNA polymerase III transcription machinery: a paradigm for eukaryotic gene activation.

Siaut M, et al. (2003 Jan). An Rpb4/Rpb7-like complex in yeast RNA polymerase III contains the orthologue of mammalian CGRP-RCP.

Jasiak AJ, et al. (2006 Jul 7). Structural biology of RNA polymerase III: subcomplex C17/25 X-ray structure and 11 subunit enzyme model.

Iwasaki O, et al. (2010 Jan 15). Centromeric localization of dispersed Pol III genes in fission yeast.

Ehara H, et al. (2011 Sep). Crystal structure of the C17/25 subcomplex from Schizosaccharomyces pombe RNA polymerase III.

Pancaldi V, et al. (2012 Apr). Predicting the fission yeast protein interaction network.

Vanoosthuyse V, et al. (2014 Jun). CPF-associated phosphatase activity opposes condensin-mediated chromosome condensation.

Legros P, et al. (2014 Nov). RNA processing factors Swd2.2 and Sen1 antagonize RNA Pol III-dependent transcription and the localization of condensin at Pol III genes.

McDonald KR, et al. (2016 Sep). Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 1 genes with posterior transmembrane prediction > 50%