FOG16089
EOG898SGW

sce:RAD53

Genes: 31

SGD Description
DNA damage response protein kinase; required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication; activates the downstream kinase Dun1p; differentially senses mtDNA depletion and mitochondrial ROS; required for regulation of copper genes in response to DNA-damaging agents; relocalizes to cytosol in response to hyoxia


PomBase Description
replication checkpoint kinase Cds1


References

Stern DF, et al. (1991 Feb). Spk1, a new kinase from Saccharomyces cerevisiae, phosphorylates proteins on serine, threonine, and tyrosine.

Zheng P, et al. (1993 Sep). SPK1 is an essential S-phase-specific gene of Saccharomyces cerevisiae that encodes a nuclear serine/threonine/tyrosine kinase.

Allen JB, et al. (1994 Oct 15). The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast.

Liao H, et al. (1999 Dec 10). Structure and function of a new phosphopeptide-binding domain containing the FHA2 of Rad53.

Pellicioli A, et al. (1999 Nov 15). Activation of Rad53 kinase in response to DNA damage and its effect in modulating phosphorylation of the lagging strand DNA polymerase.

Sanchez Y, et al. (1999 Nov 5). Control of the DNA damage checkpoint by chk1 and rad53 protein kinases through distinct mechanisms.

Liao H, et al. (2000 Dec 15). Structure of the FHA1 domain of yeast Rad53 and identification of binding sites for both FHA1 and its target protein Rad9.

Durocher D, et al. (2000 Nov). The molecular basis of FHA domain:phosphopeptide binding specificity and implications for phospho-dependent signaling mechanisms.

Kim EM, et al. (2002 Feb 1). Phosphorylation of Rph1, a damage-responsive repressor of PHR1 in Saccharomyces cerevisiae, is dependent upon Rad53 kinase.

Pike BL, et al. (2004 Apr). Mdt1, a novel Rad53 FHA1 domain-interacting protein, modulates DNA damage tolerance and G(2)/M cell cycle progression in Saccharomyces cerevisiae.

Chi A, et al. (2007 Feb 13). Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.

Van Damme P, et al. (2012 Jul 31). N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 22 genes with posterior transmembrane prediction > 50%