FOG13549
EOG8HT77M

sce:PUF6

Genes: 29

SGD Description
Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis


PomBase Description
Puf family RNA-binding protein Puf6 (predicted)


References

Gu W, et al. (2004 Jun 15). A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization.

Chi A, et al. (2007 Feb 13). Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.

Deng Y, et al. (2008 Apr 15). Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation.

Zheng W, et al. (2008 Nov). Nonsense-mediated decay of ash1 nonsense transcripts in Saccharomyces cerevisiae.

Shen Z, et al. (2009 Apr). Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p.

Van Damme P, et al. (2012 Jul 31). N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 2 genes with posterior transmembrane prediction > 50%