FOG11279
EOG8QJQ6Q

sce:HYP2;ANB1

Genes: 34

SGD Description
Translation elongation factor eIF-5A; required for translation of proteins containing polyproline stretches, including Bni1p, and this leads to a requirement for mating projection formation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication|Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication


PomBase Description
translation elongation factor eIF5A (predicted)


AspGD Description
Ortholog(s) have ribosome binding, translation elongation factor activity, translation initiation factor activity


References

Benne R, et al. (1978 May 10). The mechanism of action of protein synthesis initiation factors from rabbit reticulocytes.

Lowry CV, et al. (1986 Dec). Negative regulation of the Saccharomyces cerevisiae ANB1 gene by heme, as mediated by the ROX1 gene product.

Gordon ED, et al. (1987 Dec 5). Hypusine formation in eukaryotic initiation factor 4D is not reversed when rates or specificity of protein synthesis is altered.

Lowry CV, et al. (1988 Nov). ROX1 encodes a heme-induced repression factor regulating ANB1 and CYC7 of Saccharomyces cerevisiae.

Trueblood CE, et al. (1988 Nov). Identification of REO1, a gene involved in negative regulation of COX5b and ANB1 in aerobically grown Saccharomyces cerevisiae.

Mehta KD, et al. (1989 May 25). Identification of an upstream repressor site controlling the expression of an anaerobic gene (ANB1) in Saccharomyces cerevisiae.

Kitaoka Y, et al. (1990). Sequence determination of cDNA encoding yeast cycloheximide sensitivity factor (CH-SF) and its plausible role at a first peptide bond formation step in the initiation process of protein synthesis.

Mehta KD, et al. (1990 May 25). The ANB1 locus of Saccharomyces cerevisiae encodes the protein synthesis initiation factor eIF-4D.

Lowry CV, et al. (1990 Nov). A hypoxic consensus operator and a constitutive activation region regulate the ANB1 gene of Saccharomyces cerevisiae.

Schnier J, et al. (1991 Jun). Translation initiation factor 5A and its hypusine modification are essential for cell viability in the yeast Saccharomyces cerevisiae.

Kang HA, et al. (1993 Jul 15). Translation initiation factor eIF-5A, the hypusine-containing protein, is phosphorylated on serine in Saccharomyces cerevisiae.

Schwelberger HG, et al. (1993 Jul 5). Translation initiation factor eIF-5A expressed from either of two yeast genes or from human cDNA. Functional identity under aerobic and anaerobic conditions.

Klier H, et al. (1993 Nov 22). Determination and mutational analysis of the phosphorylation site in the hypusine-containing protein Hyp2p.

Kang HA, et al. (1994 Feb 11). Effect of initiation factor eIF-5A depletion on protein synthesis and proliferation of Saccharomyces cerevisiae.

Zuk D, et al. (1998 May 15). A single amino acid substitution in yeast eIF-5A results in mRNA stabilization.

Lee YB, et al. (1999 May 15). Complex formation between deoxyhypusine synthase and its protein substrate, the eukaryotic translation initiation factor 5A (eIF5A) precursor.

Thompson GM, et al. (2003 Dec 18). Mapping eIF5A binding sites for Dys1 and Lia1: in vivo evidence for regulation of eIF5A hypusination.

Zanelli CF, et al. (2005 Dec). Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant.

Gruhler A, et al. (2005 Mar). Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.

Jao DL, et al. (2006 Feb 15). Tandem affinity purification revealed the hypusine-dependent binding of eukaryotic initiation factor 5A to the translating 80S ribosomal complex.

Chatterjee I, et al. (2006 Mar). Rapid depletion of mutant eukaryotic initiation factor 5A at restrictive temperature reveals connections to actin cytoskeleton and cell cycle progression.

Zanelli CF, et al. (2006 Oct 6). eIF5A binds to translational machinery components and affects translation in yeast.

Dias CA, et al. (2008 Apr). Structural modeling and mutational analysis of yeast eukaryotic translation initiation factor 5A reveal new critical residues and reinforce its involvement in protein synthesis.

Shirai A, et al. (2008 Apr 18). Global analysis of gel mobility of proteins and its use in target identification.

Wilson-Grady JT, et al. (2008 Mar). Phosphoproteome analysis of fission yeast.

Gentz PM, et al. (2009 Feb). Dimerization of the yeast eukaryotic translation initiation factor 5A requires hypusine and is RNA dependent.

Roberts-Galbraith RH, et al. (2009 Jan 12). The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring.

Gregio AP, et al. (2009 Mar 20). eIF5A has a function in the elongation step of translation in yeast.

Saini P, et al. (2009 May 7). Hypusine-containing protein eIF5A promotes translation elongation.

Ren L, et al. (2011 Feb 28). Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.

Zhang K, et al. (2011 Mar 25). Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA.

Starita LM, et al. (2012 Jan). Sites of ubiquitin attachment in Saccharomyces cerevisiae.

Van Damme P, et al. (2012 Jul 31). N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

Martins I, et al. (2013 Dec 6). Proteomic alterations induced by ionic liquids in Aspergillus nidulans and Neurospora crassa.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Sideri T, et al. (2014 Dec 1). Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.

Beckley JR, et al. (2015 Dec). A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Lee J, et al. (2017 Feb 20). Chromatin remodeller Fun30^Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%