FOG02311
EOG8WDBV2

sce:PUS1

Genes: 35

SGD Description
tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication


PomBase Description
tRNA pseudouridine synthase Lsp1|tRNA pseudouridine synthase Pus2 (predicted)


AspGD Description
Has domain(s) with predicted RNA binding, pseudouridine synthase activity and role in pseudouridine synthesis


References

Simos G, et al. (1996 May 1). Nuclear pore proteins are involved in the biogenesis of functional tRNA.

Motorin Y, et al. (1998 Jul). The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity.

Arluison V, et al. (1998 May 19). Transfer RNA-pseudouridine synthetase Pus1 of Saccharomyces cerevisiae contains one atom of zinc essential for its native conformation and tRNA recognition.

Hellmuth K, et al. (2000 Dec 1). Cloning and characterization of the Schizosaccharomyces pombe tRNA:pseudouridine synthase Pus1p.

Grandier-Vazeille X, et al. (2001 Aug 21). Yeast mitochondrial dehydrogenases are associated in a supramolecular complex.

Chen D, et al. (2003 Jan). Global transcriptional responses of fission yeast to environmental stress.

Wilkinson CR, et al. (2004 Dec 29). Ubiquitin-like protein Hub1 is required for pre-mRNA splicing and localization of an essential splicing factor in fission yeast.

Jossé L, et al. (2006 Jan 1). Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis.

Wilson-Grady JT, et al. (2008 Mar). Phosphoproteome analysis of fission yeast.

Takeda K, et al. (2011). Identification of genes affecting the toxicity of anti-cancer drug bortezomib by genome-wide screening in S. pombe.

Medina B, et al. (2012 Dec 7). The ubiquitin-associated (UBA) 1 domain of Schizosaccharomyces pombe Rhp23 is essential for the recognition of ubiquitin-proteasome system substrates both in vitro and in vivo.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Mojardín L, et al. (2015). Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.

Hu L, et al. (2016 Aug). Genetic evidence for involvement of membrane trafficking in the action of 5-fluorouracil.

Dudin O, et al. (2017 Apr). A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.

Lee J, et al. (2017 Feb 20). Chromatin remodeller Fun30^Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Deryusheva S, et al. (2017 Jul). Dual nature of pseudouridylation in U2 snRNA: Pus1p-dependent and Pus1p-independent activities in yeasts and higher eukaryotes.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 2 genes with posterior transmembrane prediction > 50%

FOG02312
EOG8WDBV2

sce:PUS2

Genes: 2

SGD Description
Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification; mutation also affects pseudouridylation of some nuclear-encoded mRNAs; PUS2 has a paralog, PUS1, that arose from the whole genome duplication


PomBase Description
tRNA pseudouridine synthase Pus3 (predicted)


References

Simos G, et al. (1996 May 1). Nuclear pore proteins are involved in the biogenesis of functional tRNA.

Feuermann M, et al. (1997 Jul). The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes.

Behm-Ansmant I, et al. (2007 Oct). The Saccharomyces cerevisiae Pus2 protein encoded by YGL063w ORF is a mitochondrial tRNA:Psi27/28-synthase.

Guydosh NR, et al. (2017 Sep 25). Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in <i>S. pombe</i>.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%