FOG01565
EOG8G4F7M

sce:URA3

Genes: 32

SGD Description
Orotidine-5'-phosphate (OMP) decarboxylase; catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound


PomBase Description
orotidine 5'-phosphate decarboxylase Ura4


AspGD Description
Orotidine-5'-phosphate decarboxylase; mutants show decreased resistance to weak acid preservatives


References

Palmer LM, et al. (1975 Jun 19). Pyrimidine biosynthesis in Aspergillus nidulans: isolation and preliminary characterisation of auxotrophic mutants.

Kohli J, et al. (1977 Nov). Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.

Rose M, et al. (1983 Nov 15). Structure and function of the yeast URA3 gene. Differentially regulated expression of hybrid beta-galactosidase from overlapping coding sequences in yeast.

Rose M, et al. (1984 Jul-Aug). Structure and function of the yeast URA3 gene: expression in Escherichia coli.

Ballance DJ, et al. (1985). Development of a high-frequency transforming vector for Aspergillus nidulans.

Barnes DE, et al. (1986). Behaviour of recombinant plasmids in Aspergillus nidulans: structure and stability.

Yarger JG, et al. (1986 Apr). Transcription terminator-like element within a Saccharomyces cerevisiae promoter region.

Bach ML, et al. (1987). Cloning and expression of the OMP decarboxylase gene URA4 from Schizosaccharomyces pombe.

Goosen T, et al. (1987). Transformation of Aspergillus niger using the homologous orotidine-5'-phosphate-decarboxylase gene.

Oakley BR, et al. (1987). Cloning, mapping and molecular analysis of the pyrG (orotidine-5'-phosphate decarboxylase) gene of Aspergillus nidulans.

Shuster JR, et al. (1987 Oct 26). Sequence of the Kluyveromyces lactis URA3 gene.

Radford A, et al. (1988 Aug). Comparison of the orotidine 5'-monophosphate decarboxylase sequences of eight species.

Waring RB, et al. (1989 Jun 30). Characterization of an inducible expression system in Aspergillus nidulans using alcA and tubulin-coding genes.

Losberger C, et al. (1989 Sep). Sequence and transcript analysis of the C. albicans URA3 gene encoding orotidine-5'-phosphate decarboxylase.

Ponticelli AS, et al. (1989 Sep). Meiotic recombination-deficient mutants of Schizosaccharomyces pombe.

de Ruiter-Jacobs YM, et al. (1989 Sep). A gene transfer system based on the homologous pyrG gene and efficient expression of bacterial genes in Aspergillus oryzae.

Oza K, et al. (1990 Jun). Cloning of the DNA repair gene, uvsF, by transformation of Aspergillus nidulans.

Lloyd AT, et al. (1991 Nov). Codon usage in Aspergillus nidulans.

Girard JP, et al. (1993 Apr 25). Study of multiple fibrillarin mRNAs reveals that 3' end formation in Schizosaccharomyces pombe is sensitive to cold shock.

Holt CL, et al. (1993 Oct 29). A novel phage lambda replacement Cre-lox vector that has automatic subcloning capabilities.

Varga J, et al. (1994 Apr). Assignment of RFLP, RAPD and isoenzyme markers to Aspergillus nidulans chromosomes, using chromosome-substituted segregants of a hybrid of A. nidulans and A. quadrilineatus.

Yang VW, et al. (1994 Dec). High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2.

Bowyer P, et al. (1994 Feb). An "instant gene bank" method for heterologous gene cloning: complementation of two Aspergillus nidulans mutants with Gaeumannomyces graminis DNA.

Zhou P, et al. (1994 May 3). A system for gene cloning and manipulation in the yeast Candida glabrata.

Collett MA, et al. (1995 May 26). A mutualistic fungal symbiont of perennial ryegrass contains two different pyr4 genes, both expressing orotidine-5'-monophosphate decarboxylase.

Benko Z, et al. (1997 Jun). Caffeine-resistance in S. pombe: mutations in three novel caf genes increase caffeine tolerance and affect radiation sensitivity, fertility, and cell cycle.

Clutterbuck AJ, et al. (1997 Jun). The validity of the Aspergillus nidulans linkage map.

Weidner G, et al. (1998 May). Development of a homologous transformation system for the human pathogenic fungus Aspergillus fumigatus based on the pyrG gene encoding orotidine 5'-monophosphate decarboxylase.

Aleksenko A, et al. (1999 Aug). Structural and transcriptional analysis of the pyrABCN, pyrD and pyrF genes in Aspergillus nidulans and the evolutionary origin of fungal dihydroorotases.

Davis K, et al. (1999 Sep 15). Gene disruption in Schizosaccharomyces pombe using a temperature-sensitive Ura4p.

Miller BG, et al. (2000 Feb 29). Anatomy of a proficient enzyme: the structure of orotidine 5'-monophosphate decarboxylase in the presence and absence of a potential transition state analog.

Sinnemann SJ, et al. (2000 May). Cloning and heterologous expression of Solorina crocea pyrG.

Justino A, et al. (2001 Mar). The Aspergillus nidulans pyrG89 mutation alters glycosylation of secreted acid phosphatase.

Miller BG, et al. (2001 May 4). Dissecting a charged network at the active site of orotidine-5'-phosphate decarboxylase.

Bansal PK, et al. (2001 Oct). A minisatellite sequence in the upstream region of the DURA3 gene from the halotolerant yeast Debaryomyces hansenii.

Mauersberger S, et al. (2001 Sep). Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia lipolytica: generation of tagged mutations in genes involved in hydrophobic substrate utilization.

Kim MJ, et al. (2002 Dec 31). Post-transcriptional regulation of ura4+ gene expression by glucose in Schizosaccharomyces pombe.

Poloni D, et al. (2002 Jan 30). A DMSO-sensitive conditional mutant of the fission yeast orthologue of the Saccharomyces cerevisiae SEC13 gene is defective in septation.

Didmon M, et al. (2002 Jul). Identifying regulators of pheromone signalling in the fission yeast Schizosaccharomyces pombe.

Peng J, et al. (2003 Aug). A proteomics approach to understanding protein ubiquitination.

Kanamasa S, et al. (2003 Dec). Transformation of Aspergillus aculeatus using the drug resistance gene of Aspergillus oryzae and the pyrG gene of Aspergillus nidulans.

Krappmann S, et al. (2003 Feb). Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue.

Fierro F, et al. (2004 Jan 15). High efficiency transformation of Penicillium nalgiovense with integrative and autonomously replicating plasmids.

van Slegtenhorst M, et al. (2004 Mar 26). Tsc1+ and tsc2+ regulate arginine uptake and metabolism in Schizosaccharomyces pombe.

Matsuyama A, et al. (2004 Nov). pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast.

Takahashi T, et al. (2004 Oct). Efficient gene disruption in the koji-mold Aspergillus sojae using a novel variation of the positive-negative method.

Weisman R, et al. (2005 Feb). Regulation of leucine uptake by tor1+ in Schizosaccharomyces pombe is sensitive to rapamycin.

Storms R, et al. (2005 May). Plasmid vectors for protein production, gene expression and molecular manipulations in Aspergillus niger.

van Slegtenhorst M, et al. (2005 Oct 1). Pas1, a G1 cyclin, regulates amino acid uptake and rescues a delay in G1 arrest in Tsc1 and Tsc2 mutants in Schizosaccharomyces pombe.

Osmani AH, et al. (2006). Identification and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue technique.

Forgue P, et al. (2006 Aug). NMR metabolic profiling of Aspergillus nidulans to monitor drug and protein activity.

Nayak T, et al. (2006 Mar). A versatile and efficient gene-targeting system for Aspergillus nidulans.

Kohda TA, et al. (2007 Feb). Fission yeast autophagy induced by nitrogen starvation generates a nitrogen source that drives adaptation processes.

Amillis S, et al. (2007 May-Jun). Regulation of expression and kinetic modeling of substrate interactions of a uracil transporter in Aspergillus nidulans.

Navarrete K, et al. (2009). Molecular characterization of the niaD and pyrG genes from Penicillium camemberti, and their use as transformation markers.

Shimizu M, et al. (2009 Jan). Proteomic analysis of Aspergillus nidulans cultured under hypoxic conditions.

Kitagawa T, et al. (2010 Aug). Construction of a beta-glucosidase expression system using the multistress-tolerant yeast Issatchenkia orientalis.

Robellet X, et al. (2010 Aug). Gene silencing of transgenes inserted in the Aspergillus nidulans alcM and/or alcS loci.

Meyer V, et al. (2011 Mar). Aspergillus as a multi-purpose cell factory: current status and perspectives.

Krzyzanowski MK, et al. (2012). Identification and functional analysis of the erh1(+) gene encoding enhancer of rudimentary homolog from the fission yeast Schizosaccharomyces pombe.

Van Damme P, et al. (2012 Jul 31). N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

Sabouri N, et al. (2012 Mar 15). DNA replication through hard-to-replicate sites, including both highly transcribed RNA Pol II and Pol III genes, requires the S. pombe Pfh1 helicase.

Takahashi H, et al. (2012 Nov 2). The SAGA histone acetyltransferase complex regulates leucine uptake through the Agp3 permease in fission yeast.

Matsuo Y, et al. (2013). Polypeptone induces dramatic cell lysis in ura4 deletion mutants of fission yeast.

Sun X, et al. (2013 May). PyrG is required for maintaining stable cellular uracil level and normal sporulation pattern under excess uracil stress in Aspergillus nidulans.

Das J, et al. (2013 May 21). Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Nishino K, et al. (2015). Cell Lysis in S. pombe ura4 Mutants Is Suppressed by Loss of Functional Pub1, Which Regulates the Uracil Transporter Fur4.

Beckley JR, et al. (2015 Dec). A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Nishino K, et al. (2017 Jul). Urea enhances cell lysis of Schizosaccharomyces pombe ura4 mutants.

Maksimov V, et al. (2017 May). Stress sensitivity of a fission yeast strain lacking histidine kinases is rescued by the ectopic expression of Chk1 from Candida albicans.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%