FOG00899
EOG8K3JBH
TKL1
sce:TKL1

Genes: 34

Protein description
Main ortholog, likely carries flux from PPP to glycoylsis


SGD Description
Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication


PomBase Description
transketolase (predicted)


AspGD Description
Putative transketolase; expression induced by growth on xylose; induced by growth on starch and lactate


Suggested Analysis
Investigate directionaly and origin of duplications


References

Nixon PF, et al. (1991 Dec). The N-terminal amino acid sequence of yeast transketolase.

Fletcher TS, et al. (1992 Feb 18). DNA sequence of the yeast transketolase gene.

Lindqvist Y, et al. (1992 Jul). Three-dimensional structure of transketolase, a thiamine diphosphate dependent enzyme, at 2.5 A resolution.

Sundström M, et al. (1993 Nov 15). Yeast TKL1 gene encodes a transketolase that is required for efficient glycolysis and biosynthesis of aromatic amino acids.

König S, et al. (1994 Apr 8). Specificity of coenzyme binding in thiamin diphosphate-dependent enzymes. Crystal structures of yeast transketolase in complex with analogs of thiamin diphosphate.

Nikkola M, et al. (1994 May 6). Refined structure of transketolase from Saccharomyces cerevisiae at 2.0 A resolution.

Metzger MH, et al. (1994 Nov). Isolation and characterization of the Pichia stipitis transketolase gene and expression in a xylose-utilising Saccharomyces cerevisiae transformant.

Wikner C, et al. (1995 Nov 1). His103 in yeast transketolase is required for substrate recognition and catalysis.

Wikner C, et al. (1997 Dec 16). Identification of catalytically important residues in yeast transketolase.

Jacoby JJ, et al. (1997 Jan). Analysis of a transketolase gene from Kluyveromyces lactis reveals that the yeast enzymes are more related to transketolases of prokaryotic origins than to those of higher eukaryotes.

Nilsson U, et al. (1997 Jan 17). Examination of substrate binding in thiamin diphosphate-dependent transketolase by protein crystallography and site-directed mutagenesis.

Meshalkina L, et al. (1997 Mar 1). Examination of the thiamin diphosphate binding site in yeast transketolase by site-directed mutagenesis.

Fiedler E, et al. (2002 Jan 22). Snapshot of a key intermediate in enzymatic thiamin catalysis: crystal structure of the alpha-carbanion of (alpha,beta-dihydroxyethyl)-thiamin diphosphate in the active site of transketolase from Saccharomyces cerevisiae.

Sevostyanova IA, et al. (2008 May 1). Effect of bivalent cations on the interaction of transketolase with its donor substrate.

Shimizu M, et al. (2009 Jan). Proteomic analysis of Aspergillus nidulans cultured under hypoxic conditions.

Flipphi M, et al. (2009 Mar). Biodiversity and evolution of primary carbon metabolism in Aspergillus nidulans and other Aspergillus spp.

Starita LM, et al. (2012 Jan). Sites of ubiquitin attachment in Saccharomyces cerevisiae.

Saykhedkar S, et al. (2012 Jul 26). A time course analysis of the extracellular proteome of Aspergillus nidulans growing on sorghum stover.

Van Damme P, et al. (2012 Jul 31). N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

Martins I, et al. (2013 Dec 6). Proteomic alterations induced by ionic liquids in Aspergillus nidulans and Neurospora crassa.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 15 genes with posterior transmembrane prediction > 50%

FOG00900
EOG8K3JBH
EOG8VT4CF

sce:TKL2

Genes: 2

Protein description
TKL2 ohnolog, likely carries flux from glycolysis to PPP


Parent
ohnolog:FOG00899


SGD Description
Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL2 has a paralog, TKL1, that arose from the whole genome duplication


Suggested Analysis
Investigate directionaly and origin of duplications


References

Schaaff-Gerstenschläger I, et al. (1993 Oct 1). TKL2, a second transketolase gene of Saccharomyces cerevisiae. Cloning, sequence and deletion analysis of the gene.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00901
EOG8K3JBH

sce:absent

Genes: 9

Protein description
Unknown function


Suggested Analysis
Investigate directionaly and origin of duplications

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 3 genes with posterior transmembrane prediction > 50%

FOG00902
EOG8K3JBH

sce:absent

Genes: 10

Protein description
DAS involved in methanol metabolism


AspGD Description
Dihydroxyacetone synthase


Suggested Analysis
Investigate directionaly and origin of duplications


References

Flipphi M, et al. (2009 Mar). Biodiversity and evolution of primary carbon metabolism in Aspergillus nidulans and other Aspergillus spp.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 8 genes with posterior transmembrane prediction > 50%