FOG00608
EOG8280HC
CIT0
sce:absent

Genes: 33

Protein description
Mitochondrial and peroxisomal citrate synthase


Features
[m];[x]


PomBase Description
citrate synthase Cit1


AspGD Description
Putative citrate synthase; predicted N-terminal mitochondrial import signal and a C-terminal peroxisomal target sequence (AKL)


References

Uchiyama H, et al. (1982 Jul). Subcellular localization of the methylcitric-acid-cycle enzymes in propionate metabolism of Yarrowia lipolytica.

Park BW, et al. (1997 Apr 30). Cloning and characterization of the citA gene encoding the mitochondrial citrate synthase of Aspergillus nidulans.

Casey GD, et al. (2003). Molecular detection of Candida krusei contamination in fruit juice using the citrate synthase gene cs1 and a potential role for this gene in the adaptive response to acetic acid.

Sims AH, et al. (2004 Feb). Use of expressed sequence tag analysis and cDNA microarrays of the filamentous fungus Aspergillus nidulans.

David H, et al. (2006). Metabolic network driven analysis of genome-wide transcription data from Aspergillus nidulans.

Salazar M, et al. (2009 Dec). Uncovering transcriptional regulation of glycerol metabolism in Aspergilli through genome-wide gene expression data analysis.

Lee S, et al. (2009 Dec 31). Aspartyl aminopeptidase of Schizosaccharomyces pombe has a molecular chaperone function.

Shimizu M, et al. (2009 Jan). Proteomic analysis of Aspergillus nidulans cultured under hypoxic conditions.

Flipphi M, et al. (2009 Mar). Biodiversity and evolution of primary carbon metabolism in Aspergillus nidulans and other Aspergillus spp.

Min IS, et al. (2010 Apr). Differential expression of citA gene encoding the mitochondrial citrate synthase of Aspergillus nidulans in response to developmental status and carbon sources.

Murray SL, et al. (2010 Apr). Metabolic and developmental effects resulting from deletion of the citA gene encoding citrate synthase in Aspergillus nidulans.

Masuo S, et al. (2010 Dec). Global gene expression analysis of Aspergillus nidulans reveals metabolic shift and transcription suppression under hypoxia.

Rhind N, et al. (2011 May 20). Comparative functional genomics of the fission yeasts.

Etxebeste O, et al. (2012). GmcA is a putative glucose-methanol-choline oxidoreductase required for the induction of asexual development in Aspergillus nidulans.

Sun LL, et al. (2013). Global analysis of fission yeast mating genes reveals new autophagy factors.

Szilágyi M, et al. (2013 Jan). Transcriptome changes initiated by carbon starvation in Aspergillus nidulans.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Beckley JR, et al. (2015 Dec). A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Guo L, et al. (2016 Oct 13). Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.

Dudin O, et al. (2017 Apr). A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 7 genes with posterior transmembrane prediction > 50%

FOG00609
EOG8280HC
CIT1
sce:CIT1

Genes: 1

Protein description
Mitochondrial citrate synthase


Features
[m]


Parent
ortholog?:FOG00608


SGD Description
Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication


References

Tracy JW, et al. (1975 May). Reversible, coenzyme-A-mediated inactivation of biosynthetic condensing enzymes in yeast: a possible regulatory mechanism.

Suissa M, et al. (1984 Aug). Isolation of the nuclear yeast genes for citrate synthase and fifteen other mitochondrial proteins by a new screening method.

Rosenkrantz M, et al. (1986 Dec). Mitochondrial and nonmitochondrial citrate synthases in Saccharomyces cerevisiae are encoded by distinct homologous genes.

Rickey TM, et al. (1986 Feb). Extramitochondrial citrate synthase activity in bakers' yeast.

Kim KS, et al. (1986 Jun). Saccharomyces cerevisiae contains two functional citrate synthase genes.

Brent LG, et al. (1987 Jan 5). The interaction of yeast citrate synthase with yeast mitochondrial inner membranes.

Kispal G, et al. (1988 Aug 15). Metabolic changes in Saccharomyces cerevisiae strains lacking citrate synthases.

Lewin AS, et al. (1990 Apr). Citrate synthase encoded by the CIT2 gene of Saccharomyces cerevisiae is peroxisomal.

Grigorenko EV, et al. (1990 Oct-Dec). Citrate synthase 1 interacts with the citrate transporter of yeast mitochondria.

Kispal G, et al. (1991 Apr). Studies on yeast peroxisomal citrate synthase.

Liao XS, et al. (1991 Jan). Intramitochondrial functions regulate nonmitochondrial citrate synthase (CIT2) expression in Saccharomyces cerevisiae.

Singh KK, et al. (1992 Dec). Alternative topogenic signals in peroxisomal citrate synthase of Saccharomyces cerevisiae.

Lalo D, et al. (1994 Apr). Organization of the centromeric region of chromosome XIV in Saccharomyces cerevisiae.

Huang ME, et al. (1994 Jun). Revised nucleotide sequence of the COR region of yeast Saccharomyces cerevisiae chromosome X.

Jia YK, et al. (1997 Apr). The CIT3 gene of Saccharomyces cerevisiae encodes a second mitochondrial isoform of citrate synthase.

Dix I, et al. (1999 Mar). The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae.

Ben-Yehuda S, et al. (2000 Dec). Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae.

Grandier-Vazeille X, et al. (2001 Aug 21). Yeast mitochondrial dehydrogenases are associated in a supramolecular complex.

Chen CH, et al. (2001 Jan 5). Identification and characterization of two novel components of the Prp19p-associated complex, Ntc30p and Ntc20p.

Ohi MD, et al. (2002 Apr). Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs.

Chen CH, et al. (2002 Feb 15). Functional and physical interactions between components of the Prp19p-associated complex.

Ohi MD, et al. (2002 Jun). Characterization of interactions among the Cef1p-Prp19p-associated splicing complex.

Dahan O, et al. (2002 Oct 15). Mutations in genes of Saccharomyces cerevisiae encoding pre-mRNA splicing factors cause cell cycle arrest through activation of the spindle checkpoint.

Graybill ER, et al. (2007 Sep 1). Functional comparison of citrate synthase isoforms from S. cerevisiae.

Van Damme P, et al. (2012 Jul 31). N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00610
EOG8280HC
CIT2
sce:CIT2

Genes: 1

Protein description
Peroxisomal citrate synthase, also has [c] localization


Features
[x];[c]


Parent
paralog:FOG00608


SGD Description
Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication


References

Rosenkrantz M, et al. (1986 Dec). Mitochondrial and nonmitochondrial citrate synthases in Saccharomyces cerevisiae are encoded by distinct homologous genes.

Liao XS, et al. (1991 Jan). Intramitochondrial functions regulate nonmitochondrial citrate synthase (CIT2) expression in Saccharomyces cerevisiae.

Peng J, et al. (2003 Aug). A proteomics approach to understanding protein ubiquitination.

Starita LM, et al. (2012 Jan). Sites of ubiquitin attachment in Saccharomyces cerevisiae.

Van Damme P, et al. (2012 Jul 31). N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00611
EOG8280HC
CIT3
sce:CIT3

Genes: 15

Protein description
Mitochondrial dual specificity mitochondrial citrate and methylcitrate synthase


Features
[m]


Parent
ortholog:FOG00608


SGD Description
Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate


AspGD Description
2-methylcitrate synthase, component of the methyl citric acid cycle


References

Tracy JW, et al. (1975 May). Reversible, coenzyme-A-mediated inactivation of biosynthetic condensing enzymes in yeast: a possible regulatory mechanism.

Jia YK, et al. (1997 Apr). The CIT3 gene of Saccharomyces cerevisiae encodes a second mitochondrial isoform of citrate synthase.

Lee JG, et al. (2000 Dec). Identification of a cryptic N-terminal signal in Saccharomyces cerevisiae peroxisomal citrate synthase that functions in both peroxisomal and mitochondrial targeting.

Brock M, et al. (2000 Mar). Methylcitrate synthase from Aspergillus nidulans: implications for propionate as an antifungal agent.

Hiltunen JK, et al. (2003 Apr). The biochemistry of peroxisomal beta-oxidation in the yeast Saccharomyces cerevisiae.

Zhang YQ, et al. (2004 Apr). Blockage of methylcitrate cycle inhibits polyketide production in Aspergillus nidulans.

Brock M, et al. (2004 Aug). On the mechanism of action of the antifungal agent propionate.

Zhang YQ, et al. (2004 Oct). Connection of propionyl-CoA metabolism to polyketide biosynthesis in Aspergillus nidulans.

David H, et al. (2006). Metabolic network driven analysis of genome-wide transcription data from Aspergillus nidulans.

Graybill ER, et al. (2007 Sep 1). Functional comparison of citrate synthase isoforms from S. cerevisiae.

Fleck CB, et al. (2008 May). Characterization of an acyl-CoA: carboxylate CoA-transferase from Aspergillus nidulans involved in propionyl-CoA detoxification.

Flipphi M, et al. (2009 Mar). Biodiversity and evolution of primary carbon metabolism in Aspergillus nidulans and other Aspergillus spp.

Murray SL, et al. (2010 Apr). Metabolic and developmental effects resulting from deletion of the citA gene encoding citrate synthase in Aspergillus nidulans.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 3 genes with posterior transmembrane prediction > 50%