FOG00231
EOG8BNZT3

sce:PAP2;TRF5

Genes: 35

Protein description
RNA polymerase


SGD Description
Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of TRAMP; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; required for mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism; overlapping but non-redundant functions with Trf5p; relocalizes to cytosol in response to hypoxia|Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p


PomBase Description
poly(A) polymerase Cid14


AspGD Description
Ortholog(s) have polynucleotide adenylyltransferase activity, ribosomal large subunit binding activity


References

Sasnauskas K, et al. (1992 Apr). Molecular cloning and analysis of autonomous replicating sequence of Candida maltosa.

Bossier P, et al. (1993 Nov 5). Overexpression of YAP2, coding for a new yAP protein, and YAP1 in Saccharomyces cerevisiae alleviates growth inhibition caused by 1,10-phenanthroline.

Wu A, et al. (1993 Sep 5). Yeast bZip proteins mediate pleiotropic drug and metal resistance.

Hirata D, et al. (1994 Feb). Stress-induced transcriptional activation mediated by YAP1 and YAP2 genes that encode the Jun family of transcriptional activators in Saccharomyces cerevisiae.

Stephen DW, et al. (1995 May). The role of the YAP1 and YAP2 genes in the regulation of the adaptive oxidative stress responses of Saccharomyces cerevisiae.

Sadoff BU, et al. (1995 Oct). Isolation of mutants of Saccharomyces cerevisiae requiring DNA topoisomerase I.

Castaño IB, et al. (1996 Jun 15). A novel family of TRF (DNA topoisomerase I-related function) genes required for proper nuclear segregation.

Castaño IB, et al. (1996 Oct 15). Mitotic chromosome condensation in the rDNA requires TRF4 and DNA topoisomerase I in Saccharomyces cerevisiae.

Kuge S, et al. (1997 Apr 1). Regulation of yAP-1 nuclear localization in response to oxidative stress.

Fernandes L, et al. (1997 Dec). Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions.

Vilela C, et al. (1999 Jun 1). Post-termination ribosome interactions with the 5'UTR modulate yeast mRNA stability.

Walowsky C, et al. (1999 Mar 12). The topoisomerase-related function gene TRF4 affects cellular sensitivity to the antitumor agent camptothecin.

Wang Z, et al. (2000 Aug 4). Pol kappa: A DNA polymerase required for sister chromatid cohesion.

Ayad-Durieux Y, et al. (2000 Dec). A PAK-like protein kinase is required for maturation of young hyphae and septation in the filamentous ascomycete Ashbya gossypii.

Burgers PM, et al. (2001 Nov 23). Eukaryotic DNA polymerases: proposal for a revised nomenclature.

Wang Z, et al. (2002 Feb). Structure/function analysis of the Saccharomyces cerevisiae Trf4/Pol sigma DNA polymerase.

Cohen BA, et al. (2002 May). Discrimination between paralogs using microarray analysis: application to the Yap1p and Yap2p transcriptional networks.

Saitoh S, et al. (2002 May 31). Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA.

Edwards S, et al. (2003 Apr). Saccharomyces cerevisiae DNA polymerase epsilon and polymerase sigma interact physically and functionally, suggesting a role for polymerase epsilon in sister chromatid cohesion.

Kellis M, et al. (2003 May 15). Sequencing and comparison of yeast species to identify genes and regulatory elements.

Goldman GH, et al. (2004 Apr). Aspergillus nidulans as a model system to characterize the DNA damage response in eukaryotes.

Kadaba S, et al. (2004 Jun 1). Nuclear surveillance and degradation of hypomodified initiator tRNAMet in S. cerevisiae.

Bilsland E, et al. (2004 Sep). Rck1 and Rck2 MAPKAP kinases and the HOG pathway are required for oxidative stress resistance.

Vanácová S, et al. (2005 Jun). A new yeast poly(A) polymerase complex involved in RNA quality control.

LaCava J, et al. (2005 Jun 3). RNA degradation by the exosome is promoted by a nuclear polyadenylation complex.

Wyers F, et al. (2005 Jun 3). Cryptic pol II transcripts are degraded by a nuclear quality control pathway involving a new poly(A) polymerase.

Haracska L, et al. (2005 Nov). Trf4 and Trf5 proteins of Saccharomyces cerevisiae exhibit poly(A) RNA polymerase activity but no DNA polymerase activity.

Houseley J, et al. (2006 Feb). Yeast Trf5p is a nuclear poly(A) polymerase.

Egecioglu DE, et al. (2006 Jan). Contributions of Trf4p- and Trf5p-dependent polyadenylation to the processing and degradative functions of the yeast nuclear exosome.

Kadaba S, et al. (2006 Mar). Nuclear RNA surveillance in Saccharomyces cerevisiae: Trf4p-dependent polyadenylation of nascent hypomethylated tRNA and an aberrant form of 5S rRNA.

Azevedo D, et al. (2007 Jan 23). The S. cerevisiae Yap1 and Yap2 transcription factors share a common cadmium-sensing domain.

Reis CC, et al. (2007 Mar). Contribution of Trf4/5 and the nuclear exosome to genome stability through regulation of histone mRNA levels in Saccharomyces cerevisiae.

Granato DC, et al. (2008 Aug). Nop53p interacts with 5.8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome.

Iwai K, et al. (2010 Apr 2). Peroxiredoxin Ahp1 acts as a receptor for alkylhydroperoxides to induce disulfide bond formation in the Cad1 transcription factor.

Hamill S, et al. (2010 Aug 24). Structure and function of the polymerase core of TRAMP, a RNA surveillance complex.

Morozov IY, et al. (2012 Jul). mRNA 3' tagging is induced by nonsense-mediated decay and promotes ribosome dissociation.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 28 genes with posterior transmembrane prediction > 50%

FOG00232
EOG8BNZT3

sce:absent

Genes: 1

PomBase Description
poly(A) polymerase Cid12


References

Pidoux AL, et al. (2000 Dec). Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast.

Wang SW, et al. (2000 May). Cid1, a fission yeast protein required for S-M checkpoint control when DNA polymerase delta or epsilon is inactivated.

Motamedi MR, et al. (2004 Dec 17). Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs.

Win TZ, et al. (2006 Jun). Fission yeast Cid12 has dual functions in chromosome segregation and checkpoint control.

Win TZ, et al. (2006 Mar). Requirement of fission yeast Cid14 in polyadenylation of rRNAs.

Han TX, et al. (2010). Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Gerace EL, et al. (2010 Aug 13). The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation.

Halic M, et al. (2010 Feb 19). Dicer-independent primal RNAs trigger RNAi and heterochromatin formation.

Reddy BD, et al. (2011 Feb 1). Elimination of a specific histone H3K14 acetyltransferase complex bypasses the RNAi pathway to regulate pericentric heterochromatin functions.

Bah A, et al. (2012 Apr). The telomeric transcriptome of Schizosaccharomyces pombe.

Hayashi A, et al. (2012 Apr 17). Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.

Rougemaille M, et al. (2012 Jul 10). Ers1 links HP1 to RNAi.

Sun LL, et al. (2013). Global analysis of fission yeast mating genes reveals new autophagy factors.

Buscaino A, et al. (2013 May 2). Distinct roles for Sir2 and RNAi in centromeric heterochromatin nucleation, spreading and maintenance.

Bayne EH, et al. (2014). A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Graml V, et al. (2014 Oct 27). A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.

Mojardín L, et al. (2015). Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.

Burr R, et al. (2016 Jun 3). Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast.

Malecki M, et al. (2016 Nov 25). Functional and regulatory profiling of energy metabolism in fission yeast.

Mutazono M, et al. (2017 Feb). The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%