FOG18416
EOG834TN0

sce:BUB1;MAD3

Genes: 38

SGD Description
Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication|Subunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication


PomBase Description
mitotic spindle checkpoint kinase Bub1|mitotic spindle checkpoint protein Mad3


AspGD Description
Ortholog(s) have histone kinase activity (H2A-S121 specific) activity


References

Roberts BT, et al. (1994 Dec). The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase.

Efimov VP, et al. (1998 May). A screen for dynein synthetic lethals in Aspergillus nidulans identifies spindle assembly checkpoint genes and other genes involved in mitosis.

Brady DM, et al. (2000 Jun 1). Complex formation between Mad1p, Bub1p and Bub3p is crucial for spindle checkpoint function.

Hardwick KG, et al. (2000 Mar 6). MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p.

Fraschini R, et al. (2001 Dec 3). Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores.

Kitagawa K, et al. (2003 May). Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint.

Osmani SA, et al. (2004 Apr). The early impact of genetics on our understanding of cell cycle regulation in Aspergillus nidulans.

Poddar A, et al. (2005 May). Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae.

De Souza CP, et al. (2013). Functional analysis of the Aspergillus nidulans kinome.

De Souza CP, et al. (2014). Application of a new dual localization-affinity purification tag reveals novel aspects of protein kinase biology in Aspergillus nidulans.

Edgerton H, et al. (2015 Feb). Spatial regulation of the spindle assembly checkpoint and anaphase-promoting complex in Aspergillus nidulans.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 8 genes with posterior transmembrane prediction > 50%