FOG13202
EOG8JQ2CG

sce:GCN2

Genes: 34

SGD Description
Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control


PomBase Description
eIF2 alpha kinase Gcn2


AspGD Description
Ortholog(s) have eukaryotic translation initiation factor 2alpha kinase activity, tRNA binding activity


References

Roussou I, et al. (1988 May). Transcriptional-translational regulatory circuit in Saccharomyces cerevisiae which involves the GCN4 transcriptional activator and the GCN2 protein kinase.

Wek RC, et al. (1989 Jun). Juxtaposition of domains homologous to protein kinases and histidyl-tRNA synthetases in GCN2 protein suggests a mechanism for coupling GCN4 expression to amino acid availability.

Wek RC, et al. (1990 Jun). Identification of positive-acting domains in GCN2 protein kinase required for translational activation of GCN4 expression.

Ramirez M, et al. (1991 Jun). Ribosome association of GCN2 protein kinase, a translational activator of the GCN4 gene of Saccharomyces cerevisiae.

Ramirez M, et al. (1992 Dec). Mutations activating the yeast eIF-2 alpha kinase GCN2: isolation of alleles altering the domain related to histidyl-tRNA synthetases.

Dever TE, et al. (1992 Feb 7). Phosphorylation of initiation factor 2 alpha by protein kinase GCN2 mediates gene-specific translational control of GCN4 in yeast.

Wek SA, et al. (1995 Aug). The histidyl-tRNA synthetase-related sequence in the eIF-2 alpha protein kinase GCN2 interacts with tRNA and is required for activation in response to starvation for different amino acids.

Vazquez de Aldana CR, et al. (1995 Jul 3). GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2 alpha kinase GCN2 in amino acid-starved cells.

Zhu S, et al. (1996 Oct 4). Histidyl-tRNA synthetase-related sequences in GCN2 protein kinase regulate in vitro phosphorylation of eIF-2.

Romano PR, et al. (1998 Apr). Autophosphorylation in the activation loop is required for full kinase activity in vivo of human and yeast eukaryotic initiation factor 2alpha kinases PKR and GCN2.

Zhu S, et al. (1998 Jan 16). Ribosome-binding domain of eukaryotic initiation factor-2 kinase GCN2 facilitates translation control.

Qiu H, et al. (1998 May). Dimerization by translation initiation factor 2 kinase GCN2 is mediated by interactions in the C-terminal ribosome-binding region and the protein kinase domain.

Garcia-Barrio M, et al. (2000 Apr 17). Association of GCN1-GCN20 regulatory complex with the N-terminus of eIF2alpha kinase GCN2 is required for GCN2 activation.

Dong J, et al. (2000 Aug). Uncharged tRNA activates GCN2 by displacing the protein kinase moiety from a bipartite tRNA-binding domain.

Sattlegger E, et al. (2000 Dec 1). Separate domains in GCN1 for binding protein kinase GCN2 and ribosomes are required for GCN2 activation in amino acid-starved cells.

Kubota H, et al. (2000 Jul 7). GI domain-mediated association of the eukaryotic initiation factor 2alpha kinase GCN2 with its activator GCN1 is required for general amino acid control in budding yeast.

Qiu H, et al. (2001 Mar 15). The tRNA-binding moiety in GCN2 contains a dimerization domain that interacts with the kinase domain and is required for tRNA binding and kinase activation.

Kubota H, et al. (2001 May 18). Budding yeast GCN1 binds the GI domain to activate the eIF2alpha kinase GCN2.

Padyana AK, et al. (2005 Aug 12). Structural basis for autoinhibition and mutational activation of eukaryotic initiation factor 2alpha protein kinase GCN2.

Chi A, et al. (2007 Feb 13). Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.

Dey M, et al. (2007 Mar 2). Conserved intermolecular salt bridge required for activation of protein kinases PKR, GCN2, and PERK.

Visweswaraiah J, et al. (2011 Oct 21). Evidence that eukaryotic translation elongation factor 1A (eEF1A) binds the Gcn2 protein C terminus and inhibits Gcn2 activity.

Visweswaraiah J, et al. (2012 Nov 2). Overexpression of eukaryotic translation elongation factor 3 impairs Gcn2 protein activation.

Cambiaghi TD, et al. (2014 Jan 10). Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 14 genes with posterior transmembrane prediction > 50%