FOG12279
EOG8MW6TJ

sce:RAD18

Genes: 31

SGD Description
E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA


PomBase Description
Rad18 homolog ubiquitin protein ligase E3, Rhp18


AspGD Description
Protein with strong similarity to the UvsH protein of A. nidulans which is involved in DNA repair and recombination


References

Wright PJ, et al. (1970 Jun). Ultraviolet-light sensitive mutants of Aspergillus nidulans.

Käfer E, et al. (1986 Jul). Genetic analysis of DNA repair in Aspergillus: evidence for different types of MMS-sensitive hyperrec mutants.

Chanet R, et al. (1988 Dec 30). Potential DNA-binding domains in the RAD18 gene product of Saccharomyces cerevisiae.

Jones JS, et al. (1988 Jul 25). The Saccharomyces cerevisiae RAD18 gene encodes a protein that contains potential zinc finger domains for nucleic acid binding and a putative nucleotide binding sequence.

Babudri N, et al. (1989 May). Different action of MMS and EMS in UV-sensitive strains of Aspergillus nidulans.

Osman F, et al. (1991 Feb-Mar). Isolation and characterisation of nuv11, a mutation affecting meiotic and mitotic recombination in Aspergillus nidulans.

Benit P, et al. (1992 Feb). Sequence of the sup61-RAD18 region on chromosome III of Saccharomyces cerevisiae.

Chae SK, et al. (1993 Jul-Aug). uvsI mutants defective in UV mutagenesis define a fourth epistatic group of uvs genes in Aspergillus.

Bailly V, et al. (1994 Apr 1). Specific complex formation between yeast RAD6 and RAD18 proteins: a potential mechanism for targeting RAD6 ubiquitin-conjugating activity to DNA damage sites.

Denison SH, et al. (1994 Jan 16). Mitotic catastrophe is the mechanism of lethality for mutations that confer mutagen sensitivity in Aspergillus nidulans.

Kafer E, et al. (1994 Mar). Phenotypic and epistatic grouping of hypo- and hyper-rec mus mutants in Aspergillus.

Donnelly E, et al. (1994 Nov 28). Germinating conidiospores of Aspergillus amino acid auxotrophs are hypersensitive to heat shock, oxidative stress and DNA damage.

Donnelly E, et al. (1995 Dec 18). Quantification of DNA damage and repair in amino acid auxotrophs and UV-sensitive mutants of Aspergillus nidulans using an ELISA.

Yoon JH, et al. (1995 Jul 28). The Aspergillus uvsH gene encodes a product homologous to yeast RAD18 and Neurospora UVS-2.

Iwanejko L, et al. (1996 Mar). nuvA, an Aspergillus nidulans gene involved in DNA repair and recombination, is a homologue of Saccharomyces cerevisiae RAD18 and Neurospora crassa uvs-2.

Clutterbuck AJ, et al. (1997 Jun). The validity of the Aspergillus nidulans linkage map.

Babudri N, et al. (1997 Mar). The base analog 6-N-hydroxylaminopurine (HAP) mutagenesis is dependent on the integrity of the uvsE, uvsF and uvsB genes in Aspergillus nidulans.

Bailly V, et al. (1997 Sep 12). Yeast DNA repair proteins Rad6 and Rad18 form a heterodimer that has ubiquitin conjugating, DNA binding, and ATP hydrolytic activities.

Xiao W, et al. (2000 Aug). The Saccharomyces cerevisiae RAD6 group is composed of an error-prone and two error-free postreplication repair pathways.

Ulrich HD, et al. (2000 Jul 3). Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair.

van der Laan R, et al. (2000 Oct 1). Characterization of mRAD18Sc, a mouse homolog of the yeast postreplication repair gene RAD18.

Chiuchetta SJ, et al. (2002). Recombinagenic effect of cryptolepine in uvsH+//uvsH+ and uvsH//uvsH diploid strains of Aspergillus nidulans.

Chiuchetta SJ, et al. (2002). Vincristine induces somatic segregation, via mitotic crossing-over, in diploid cells of Aspergillus nidulans.

Goldman GH, et al. (2002 Apr). The DNA damage response in filamentous fungi.

Hoege C, et al. (2002 Sep 12). RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO.

de Padula M, et al. (2004). The post-replication repair RAD18 and RAD6 genes are involved in the prevention of spontaneous mutations caused by 7,8-dihydro-8-oxoguanine in Saccharomyces cerevisiae.

Goldman GH, et al. (2004 Apr). Aspergillus nidulans as a model system to characterize the DNA damage response in eukaryotes.

Busso C, et al. (2004 Mar). Cremophor EL stimulates mitotic recombination in uvsH//uvsH diploid strain of Aspergillus nidulans.

Branzei D, et al. (2004 Nov). Rad18/Rad5/Mms2-mediated polyubiquitination of PCNA is implicated in replication completion during replication stress.

Zhang H, et al. (2005 Nov 1). The error-free component of the RAD6/RAD18 DNA damage tolerance pathway of budding yeast employs sister-strand recombination.

Souza-Júnior SA, et al. (2007). Asexual recombination in a uvsH mutant of Aspergillus nidulans.

Chi A, et al. (2007 Feb 13). Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.

Starita LM, et al. (2012 Jan). Sites of ubiquitin attachment in Saccharomyces cerevisiae.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%