FOG11225
EOG8QRFKG

sce:MUS81

Genes: 30

SGD Description
Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates function of Mms4p-Mus81p


PomBase Description
Holliday junction resolvase subunit Mus81


AspGD Description
Ortholog(s) have crossover junction endodeoxyribonuclease activity


References

Boddy MN, et al. (2000 Dec). Damage tolerance protein Mus81 associates with the FHA1 domain of checkpoint kinase Cds1.

Interthal H, et al. (2000 Jun). MUS81 encodes a novel helix-hairpin-helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae.

Mullen JR, et al. (2001 Jan). Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae.

Chen XB, et al. (2001 Nov). Human Mus81-associated endonuclease cleaves Holliday junctions in vitro.

Boddy MN, et al. (2001 Nov 16). Mus81-Eme1 are essential components of a Holliday junction resolvase.

Kaliraman V, et al. (2001 Oct 15). Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease.

Fabre F, et al. (2002 Dec 24). Alternate pathways involving Sgs1/Top3, Mus81/ Mms4, and Srs2 prevent formation of toxic recombination intermediates from single-stranded gaps created by DNA replication.

Doe CL, et al. (2002 Sep 6). Mus81-Eme1 and Rqh1 involvement in processing stalled and collapsed replication forks.

Boddy MN, et al. (2003 Aug). Replication checkpoint kinase Cds1 regulates recombinational repair protein Rad60.

Smith GR, et al. (2003 Dec). Fission yeast Mus81.Eme1 Holliday junction resolvase is required for meiotic crossing over but not for gene conversion.

Fu Y, et al. (2003 Dec 9). Functional domains required for the Saccharomyces cerevisiae Mus81-Mms4 endonuclease complex formation and nuclear localization.

Kanoh J, et al. (2003 Feb 28). The fission yeast spSet1p is a histone H3-K4 methyltransferase that functions in telomere maintenance and DNA repair in an ATM kinase Rad3-dependent pathway.

Whitby MC, et al. (2003 Feb 28). Cleavage of model replication forks by fission yeast Mus81-Eme1 and budding yeast Mus81-Mms4.

Bastin-Shanower SA, et al. (2003 May). The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10.

de los Santos T, et al. (2003 May). The Mus81/Mms4 endonuclease acts independently of double-Holliday junction resolution to promote a distinct subset of crossovers during meiosis in budding yeast.

Gaillard PH, et al. (2003 Sep). The endogenous Mus81-Eme1 complex resolves Holliday junctions by a nick and counternick mechanism.

Osman F, et al. (2003 Sep). Generating crossovers by resolution of nicked Holliday junctions: a role for Mus81-Eme1 in meiosis.

Doe CL, et al. (2004). DNA repair by a Rad22-Mus81-dependent pathway that is independent of Rhp51.

Doe CL, et al. (2004). The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast.

Ellermeier C, et al. (2004 Dec). Swi5 acts in meiotic DNA joint molecule formation in Schizosaccharomyces pombe.

Hollingsworth NM, et al. (2004 Jan 15). The Mus81 solution to resolution: generating meiotic crossovers without Holliday junctions.

Young JA, et al. (2004 Jun). Conserved and nonconserved proteins for meiotic DNA breakage and repair in yeasts.

Morikawa H, et al. (2004 Nov). Rad62 protein functionally and physically associates with the smc5/smc6 protein complex and is required for chromosome integrity and recombination repair in fission yeast.

Noguchi E, et al. (2004 Oct). Swi1 and Swi3 are components of a replication fork protection complex in fission yeast.

Kai M, et al. (2005 Apr 15). Replication checkpoint kinase Cds1 regulates Mus81 to preserve genome integrity during replication stress.

Fricke WM, et al. (2005 Feb 3). Substrate specificity of the Saccharomyces cerevisiae Mus81-Mms4 endonuclease.

Farah JA, et al. (2005 Mar). A novel recombination pathway initiated by the Mre11/Rad50/Nbs1 complex eliminates palindromes during meiosis in Schizosaccharomyces pombe.

Ii M, et al. (2005 Oct). Roles of SGS1, MUS81, and RAD51 in the repair of lagging-strand replication defects in Saccharomyces cerevisiae.

Sheedy DM, et al. (2005 Oct). Brc1-mediated DNA repair and damage tolerance.

Martín V, et al. (2006 Jun 7). Sws1 is a conserved regulator of homologous recombination in eukaryotic cells.

Pebernard S, et al. (2006 Mar). The Nse5-Nse6 dimer mediates DNA repair roles of the Smc5-Smc6 complex.

Yokoyama M, et al. (2007 Jun 1). The novel gene mus7(+) is involved in the repair of replication-associated DNA damage in fission yeast.

Cullen JK, et al. (2007 Nov). Break-induced loss of heterozygosity in fission yeast: dual roles for homologous recombination in promoting translocations and preventing de novo telomere addition.

Roseaulin L, et al. (2008 May 7). Mus81 is essential for sister chromatid recombination at broken replication forks.

Yin L, et al. (2008 Oct). Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation.

Dovey CL, et al. (2009 Dec 3). Mms1-Mms22 complex protects genome integrity in Schizosaccharomyces pombe.

Willis N, et al. (2009 Feb). Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint.

Farah JA, et al. (2009 Jun 9). Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required for efficient meiotic recombination.

Lorenz A, et al. (2010 Apr). The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant.

Tougan T, et al. (2010 Dec 1). The Mek1 phosphorylation cascade plays a role in meiotic recombination of Schizosaccharomyces pombe.

Hyppa RW, et al. (2010 Jul 23). Crossover invariance determined by partner choice for meiotic DNA break repair.

Akai Y, et al. (2011 Dec). Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing.

Prudden J, et al. (2011 Jun). DNA repair and global sumoylation are regulated by distinct Ubc9 noncovalent complexes.

Vejrup-Hansen R, et al. (2011 Mar 7). Schizosaccharomyces pombe Mms1 channels repair of perturbed replication into Rhp51 independent homologous recombination.

Groocock LM, et al. (2012 Jan). The RecQ4 orthologue Hrq1 is critical for DNA interstrand cross-link repair and genome stability in fission yeast.

Lorenz A, et al. (2012 Jun 22). The fission yeast FANCM ortholog directs non-crossover recombination during meiosis.

Steinacher R, et al. (2012 Mar 15). The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability.

Sánchez A, et al. (2012 Nov). Replication fork collapse and genome instability in a deoxycytidylate deaminase mutant.

Wehrkamp-Richter S, et al. (2012 Oct). Meiotic DNA joint molecule resolution depends on Nse5-Nse6 of the Smc5-Smc6 holocomplex.

Guo H, et al. (2013). A quality control mechanism linking meiotic success to release of ascospores.

Schonbrun M, et al. (2013 Jul 5). TORC2 is required to maintain genome stability during S phase in fission yeast.

Li PC, et al. (2013 Mar 28). Replication fork stability is essential for the maintenance of centromere integrity in the absence of heterochromatin.

Fadden AJ, et al. (2013 Nov). A winged helix domain in human MUS81 binds DNA and modulates the endonuclease activity of MUS81 complexes.

Bellini A, et al. (2014). Fission yeast Rad52 phosphorylation restrains error prone recombination pathways.

Mastro TL, et al. (2014 Dec). Increased meiotic crossovers and reduced genome stability in absence of Schizosaccharomyces pombe Rad16 (XPF).

Lorenz A, et al. (2014 Dec 16). Rad51/Dmc1 paralogs and mediators oppose DNA helicases to limit hybrid DNA formation and promote crossovers during meiotic recombination.

Caetano C, et al. (2014 Dec 24). Tolerance of deregulated G1/S transcription depends on critical G1/S regulon genes to prevent catastrophic genome instability.

Mahyous Saeyd SA, et al. (2014 Jul). Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state.

Ding L, et al. (2014 May 28). Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.

Sánchez A, et al. (2015). Ku stabilizes replication forks in the absence of Brc1.

Mejia-Ramirez E, et al. (2015 Sep). Critical Function of γH2A in S-Phase.

Onaka AT, et al. (2016 Dec 15). Rad51 and Rad54 promote noncrossover recombination between centromere repeats on the same chromatid to prevent isochromosome formation.

Nie M, et al. (2016 Jul). Functional Crosstalk between the PP2A and SUMO Pathways Revealed by Analysis of STUbL Suppressor, razor 1-1.

Ranatunga NS, et al. (2016 Oct 13). Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.

Dudin O, et al. (2017 Apr). A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 3 genes with posterior transmembrane prediction > 50%