FOG04968
EOG8F1VKJ

sce:CDC50

Genes: 31

SGD Description
Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication


PomBase Description
CDC50 domain protein, implicated in signal transduction (predicted)


AspGD Description
Ortholog(s) have phospholipid-translocating ATPase activity and role in actin cortical patch localization, endocytosis, intracellular protein transport, phospholipid translocation


References

Radji M, et al. (2001 Feb). The cloning and characterization of the CDC50 gene family in Saccharomyces cerevisiae.

Misu K, et al. (2003 Feb). Cdc50p, a conserved endosomal membrane protein, controls polarized growth in Saccharomyces cerevisiae.

Saito K, et al. (2004 Jul). Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae.

Martín-Castellanos C, et al. (2005 Nov 22). A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events.

Kim H, et al. (2006 Jul 25). A global topology map of the Saccharomyces cerevisiae membrane proteome.

Beltrao P, et al. (2009 Jun 16). Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Han TX, et al. (2010). Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Guo Y, et al. (2014 Jul). Large scale screening of genetic interaction with sgf73(+) in fission yeast.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 31 genes with posterior transmembrane prediction > 50%

FOG04969
EOG8F1VKJ

sce:LEM3

Genes: 23

SGD Description
Membrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p


References

Kato U, et al. (2002 Oct 4). A novel membrane protein, Ros3p, is required for phospholipid translocation across the plasma membrane in Saccharomyces cerevisiae.

Hanson PK, et al. (2003 Sep 19). Lem3p is essential for the uptake and potency of alkylphosphocholine drugs, edelfosine and miltefosine.

Van Damme P, et al. (2012 Jul 31). N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 23 genes with posterior transmembrane prediction > 50%

FOG04970
EOG8F1VKJ

sce:absent

Genes: 3

SGD Description
Potential noncatalytic subunit for phospholipid translocase Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication


PomBase Description
CDC50 domain protein, implicated in signal transduction (predicted)


References

Saito K, et al. (2004 Jul). Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae.

Kim H, et al. (2006 Jul 25). A global topology map of the Saccharomyces cerevisiae membrane proteome.

Furuta N, et al. (2007 Jan). Endocytic recycling in yeast is regulated by putative phospholipid translocases and the Ypt31p/32p-Rcy1p pathway.

Dodgson J, et al. (2009 Aug). Functional genomics of adhesion, invasion, and mycelial formation in Schizosaccharomyces pombe.

Lenoir G, et al. (2009 Jul 3). Cdc50p plays a vital role in the ATPase reaction cycle of the putative aminophospholipid transporter Drs2p.

Beltrao P, et al. (2009 Jun 16). Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Deshpande GP, et al. (2009 May 1). Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.

Stewart EV, et al. (2011 Apr 22). Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.

Zhang L, et al. (2013). Genome-wide screening for genes associated with valproic acid sensitivity in fission yeast.

Anver S, et al. (2014 Aug). Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.

Beckley JR, et al. (2015 Dec). A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Zhang X, et al. (2015 Oct 9). Characterization of Tamoxifen as an Antifungal Agent Using the Yeast Schizosaccharomyces Pombe Model Organism.

Dudin O, et al. (2017 Apr). A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 3 genes with posterior transmembrane prediction > 50%