FOG04707
EOG8NK99S

sce:ORC1

Genes: 34

SGD Description
Largest subunit of the origin recognition complex; involved in directing DNA replication by binding to replication origins; also involved in transcriptional silencing; exhibits ATPase activity; ORC1 has a paralog, SIR3, that arose from the whole genome duplication


PomBase Description
origin recognition complex subunit Orc1


AspGD Description
Ortholog(s) have DNA replication origin binding activity, role in mitotic DNA replication, mitotic DNA replication checkpoint and nuclear chromatin, nuclear origin of replication recognition complex localization


References

Gavin KA, et al. (1995 Dec 8). Conserved initiator proteins in eukaryotes.

Loo S, et al. (1995 Jun). The origin recognition complex in silencing, cell cycle progression, and DNA replication.

Bell SP, et al. (1995 Nov 17). The multidomain structure of Orc1p reveals similarity to regulators of DNA replication and transcriptional silencing.

Kawasaki Y, et al. (2000 Dec). Interactions between Mcm10p and other replication factors are required for proper initiation and elongation of chromosomal DNA replication in Saccharomyces cerevisiae.

Asano T, et al. (2007 Dec). Interaction between ORC and Cdt1p of Saccharomyces cerevisiae.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 22 genes with posterior transmembrane prediction > 50%

FOG04708
EOG8NK99S

sce:CDC6

Genes: 32

SGD Description
Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress


PomBase Description
MCM loader


AspGD Description
Ortholog(s) have protein serine/threonine kinase activity and role in mitotic DNA replication checkpoint, peptidyl-serine autophosphorylation, peptidyl-threonine autophosphorylation, positive regulation of DNA replication initiation


References

Lisziewicz J, et al. (1988 Dec 23). Cloning and characterization of the Saccharomyces cerevisiae CDC6 gene.

Zhou C, et al. (1989 May 25). Molecular cloning of Saccharomyces cerevisiae CDC6 gene. Isolation, identification, and sequence analysis.

Zhou C, et al. (1990 Nov 15). CDC6 mRNA fluctuates periodically in the yeast cell cycle.

Bueno A, et al. (1992 Jun). Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division.

Piatti S, et al. (1995 Aug 1). Cdc6 is an unstable protein whose de novo synthesis in G1 is important for the onset of S phase and for preventing a 'reductional' anaphase in the budding yeast Saccharomyces cerevisiae.

Santocanale C, et al. (1996 Dec 2). ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae.

Cocker JH, et al. (1996 Jan 11). An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast.

Jong A, et al. (1996 Oct). Intracellular location of the Saccharomyces cerevisiae CDC6 gene product.

Detweiler CS, et al. (1997 Mar). Cdc6p establishes and maintains a state of replication competence during G1 phase.

Drury LS, et al. (1997 Oct 1). The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast.

Weinreich M, et al. (1999 Jan 19). The Cdc6p nucleotide-binding motif is required for loading mcm proteins onto chromatin.

Wang B, et al. (1999 Mar 19). The essential role of Saccharomyces cerevisiae CDC6 nucleotide-binding site in cell growth, DNA synthesis, and Orc1 association.

Elsasser S, et al. (1999 Oct). Phosphorylation controls timing of Cdc6p destruction: A biochemical analysis.

Calzada A, et al. (2000 Mar 31). The stability of the Cdc6 protein is regulated by cyclin-dependent kinase/cyclin B complexes in Saccharomyces cerevisiae.

Kim DH, et al. (2012 Dec 28). The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase.

Fernández-Cid A, et al. (2013 May 23). An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 3 genes with posterior transmembrane prediction > 50%

FOG04709
EOG8NK99S

sce:SIR3

Genes: 2

SGD Description
Silencing protein; interacts with Sir2p, Sir4p, and histone H3 and H4 tails to establish transcriptionally silent chromatin state; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p; C-terminus (residues 840-978) assumes variant winged helix-turn-helix (wH) fold that mediates homodimerization, which is critical for holo-SIR complex loading; SIR3 has a paralog, ORC1, that arose from the whole genome duplication


References

Shore D, et al. (1984 Dec 1). Characterization of two genes required for the position-effect control of yeast mating-type genes.

Stone EM, et al. (1996 Nov). Activation of an MAP kinase cascade leads to Sir3p hyperphosphorylation and strengthens transcriptional silencing.

Gasser SM, et al. (2001 Nov 14). The molecular biology of the SIR proteins.

Wang X, et al. (2004 Sep). Importance of the Sir3 N terminus and its acetylation for yeast transcriptional silencing.

Douglas NL, et al. (2005 Dec). Dual roles for Mcm10 in DNA replication initiation and silencing at the mating-type loci.

Hou Z, et al. (2006 May). Structure of the Sir3 protein bromo adjacent homology (BAH) domain from S. cerevisiae at 1.95 A resolution.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%