FOG04507
EOG8SBCF5

sce:RAD2

Genes: 34

SGD Description
Single-stranded DNA endonuclease; cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein


PomBase Description
DNA repair nuclease Rad13


AspGD Description
Ortholog(s) have role in nucleotide-excision repair involved in interstrand cross-link repair and nucleus localization


References

Nicolet CM, et al. (1985). The RAD2 gene of Saccharomyces cerevisiae: nucleotide sequence and transcript mapping.

Madura K, et al. (1986 Jun). Nucleotide sequence, transcript mapping, and regulation of the RAD2 gene of Saccharomyces cerevisiae.

Habraken Y, et al. (1993 Nov 25). Yeast excision repair gene RAD2 encodes a single-stranded DNA endonuclease.

Wendland J, et al. (2011 Dec). Genome evolution in the eremothecium clade of the Saccharomyces complex revealed by comparative genomics.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 7 genes with posterior transmembrane prediction > 50%

FOG04508
EOG8SBCF5

sce:RAD27

Genes: 33

SGD Description
5' to 3' exonuclease, 5' flap endonuclease; required for Okazaki fragment processing and maturation, for long-patch base-excision repair and large loop repair (LLR), ribonucleotide excision repair; member of the S. pombe RAD2/FEN1 family; relocalizes to the cytosol in response to hypoxia


PomBase Description
FEN-1 endonuclease Rad2


AspGD Description
Ortholog(s) have 5'-3' exodeoxyribonuclease activity involved in UV-damage excision repair, 5'-flap endonuclease activity, double-stranded DNA 5'-3' exodeoxyribonuclease activity, single-stranded DNA 5'-3' exodeoxyribonuclease activity


References

Harrington JJ, et al. (1994 Jun 1). Functional domains within FEN-1 and RAD2 define a family of structure-specific endonucleases: implications for nucleotide excision repair.

Reagan MS, et al. (1995 Jan). Characterization of a mutant strain of Saccharomyces cerevisiae with a deletion of the RAD27 gene, a structural homolog of the RAD2 nucleotide excision repair gene.

Zhu FX, et al. (1997 May 20). Purification and characterization of the DNA polymerase alpha associated exonuclease: the RTH1 gene product.

Gomes XV, et al. (2000 Jul 17). Two modes of FEN1 binding to PCNA regulated by DNA.

Kao HI, et al. (2002 Apr 26). Cleavage specificity of Saccharomyces cerevisiae flap endonuclease 1 suggests a double-flap structure as the cellular substrate.

Ayyagari R, et al. (2003 Jan 17). Okazaki fragment maturation in yeast. I. Distribution of functions between FEN1 AND DNA2.

Rossi ML, et al. (2006 Sep 8). Reconstituted Okazaki fragment processing indicates two pathways of primer removal.

Singh P, et al. (2007 Feb 9). Concerted action of exonuclease and Gap-dependent endonuclease activities of FEN-1 contributes to the resolution of triplet repeat sequences (CTG)n- and (GAA)n-derived secondary structures formed during maturation of Okazaki fragments.

Guo Z, et al. (2008 Jul). Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair.

Ma W, et al. (2009 Mar). The transition of closely opposed lesions to double-strand breaks during long-patch base excision repair is prevented by the coordinated action of DNA polymerase delta and Rad27/Fen1.

Kalifa L, et al. (2009 Oct 2). Evidence for a role of FEN1 in maintaining mitochondrial DNA integrity.

Van Damme P, et al. (2012 Jul 31). N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG04509
EOG8SBCF5

sce:absent

Genes: 2

AspGD Description
Has domain(s) with predicted endonuclease activity, nuclease activity, single-stranded DNA binding activity, role in DNA repair, nucleotide-excision repair and nucleus localization

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%