FOG03918
EOG8N5TBS

sce:MSH3

Genes: 34

SGD Description
Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability


PomBase Description
MutS protein homolog 3


AspGD Description
Ortholog(s) have role in gene conversion at mating-type locus, termination of copy-synthesis, maintenance of DNA repeat elements, reciprocal meiotic recombination and nucleus, site of double-strand break localization


References

New L, et al. (1993 May). The yeast gene MSH3 defines a new class of eukaryotic MutS homologues.

Marsischky GT, et al. (1996 Feb 15). Redundancy of Saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair.

Johnson RE, et al. (1996 Nov 8). Evidence for involvement of yeast proliferating cell nuclear antigen in DNA mismatch repair.

Habraken Y, et al. (1996 Sep 1). Binding of insertion/deletion DNA mismatches by the heterodimer of yeast mismatch repair proteins MSH2 and MSH3.

Sugawara N, et al. (1997 Aug 19). Role of Saccharomyces cerevisiae Msh2 and Msh3 repair proteins in double-strand break-induced recombination.

Sia EA, et al. (1997 May). Microsatellite instability in yeast: dependence on repeat unit size and DNA mismatch repair genes.

Habraken Y, et al. (1997 Oct 1). Enhancement of MSH2-MSH3-mediated mismatch recognition by the yeast MLH1-PMS1 complex.

Flores-Rozas H, et al. (1998 Oct 13). The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations.

Studamire B, et al. (1999 Nov). Separation-of-function mutations in Saccharomyces cerevisiae MSH2 that confer mismatch repair defects but do not affect nonhomologous-tail removal during recombination.

Clark AB, et al. (2000 Nov 24). Functional interaction of proliferating cell nuclear antigen with MSH2-MSH6 and MSH2-MSH3 complexes.

Kellis M, et al. (2003 May 15). Sequencing and comparison of yeast species to identify genes and regulatory elements.

Stone JE, et al. (2006 Jul). Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae.

Surtees JA, et al. (2006 Jul 14). Mismatch repair factor MSH2-MSH3 binds and alters the conformation of branched DNA structures predicted to form during genetic recombination.

Lee SD, et al. (2007 Feb 9). Saccharomyces cerevisiae MSH2-MSH3 and MSH2-MSH6 complexes display distinct requirements for DNA binding domain I in mismatch recognition.

Shell SS, et al. (2007 Jun 26). Chimeric Saccharomyces cerevisiae Msh6 protein with an Msh3 mispair-binding domain combines properties of both proteins.

Harrington JM, et al. (2007 Sep). Saccharomyces cerevisiae Msh2-Msh3 acts in repair of base-base mispairs.

Li F, et al. (2008 May 9). Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 9 genes with posterior transmembrane prediction > 50%

FOG03919
EOG8N5TBS

sce:MSH2

Genes: 33

SGD Description
Protein that binds to DNA mismatches; forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP


PomBase Description
MutS protein homolog 2


AspGD Description
Ortholog(s) have ATP binding, ATPase activity, Y-form DNA binding, double-strand/single-strand DNA junction binding, four-way junction DNA binding and guanine/thymine mispair binding, more


References

Reenan RA, et al. (1992 Dec). Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functions.

Reenan RA, et al. (1992 Dec). Isolation and characterization of two Saccharomyces cerevisiae genes encoding homologs of the bacterial HexA and MutS mismatch repair proteins.

Prolla TA, et al. (1994 Aug 19). MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast.

Marsischky GT, et al. (1996 Feb 15). Redundancy of Saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair.

Alani E, et al. (1996 Oct). The Saccharomyces cerevisiae Msh2 and Msh6 proteins form a complex that specifically binds to duplex oligonucleotides containing mismatched DNA base pairs.

Umar A, et al. (1996 Oct 4). Requirement for PCNA in DNA mismatch repair at a step preceding DNA resynthesis.

Habraken Y, et al. (1996 Sep 1). Binding of insertion/deletion DNA mismatches by the heterodimer of yeast mismatch repair proteins MSH2 and MSH3.

Sugawara N, et al. (1997 Aug 19). Role of Saccharomyces cerevisiae Msh2 and Msh3 repair proteins in double-strand break-induced recombination.

Sia EA, et al. (1997 May). Microsatellite instability in yeast: dependence on repeat unit size and DNA mismatch repair genes.

Habraken Y, et al. (1997 Oct 1). Enhancement of MSH2-MSH3-mediated mismatch recognition by the yeast MLH1-PMS1 complex.

Habraken Y, et al. (1998 Apr 17). ATP-dependent assembly of a ternary complex consisting of a DNA mismatch and the yeast MSH2-MSH6 and MLH1-PMS1 protein complexes.

Studamire B, et al. (1998 Dec). Saccharomyces cerevisiae Msh2p and Msh6p ATPase activities are both required during mismatch repair.

Flores-Rozas H, et al. (1998 Oct 13). The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations.

Drotschmann K, et al. (1999 Aug 26). Mutator phenotypes of common polymorphisms and missense mutations in MSH2.

Marsischky GT, et al. (1999 Mar 12). 'Saccharomyces cerevisiae MSH2/6 complex interacts with Holliday junctions and facilitates their cleavage by phage resolution enzymes.

Drotschmann K, et al. (1999 Mar 16). Mutator phenotypes of yeast strains heterozygous for mutations in the MSH2 gene.

Studamire B, et al. (1999 Nov). Separation-of-function mutations in Saccharomyces cerevisiae MSH2 that confer mismatch repair defects but do not affect nonhomologous-tail removal during recombination.

Ni TT, et al. (1999 Sep). MSH2 and MSH6 are required for removal of adenine misincorporated opposite 8-oxo-guanine in S. cerevisiae.

Marsischky GT, et al. (1999 Sep 17). Biochemical characterization of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 complex and mispaired bases in DNA.

Clark AB, et al. (2000 Nov 24). Functional interaction of proliferating cell nuclear antigen with MSH2-MSH6 and MSH2-MSH3 complexes.

Drotschmann K, et al. (2001 Dec 7). Asymmetric recognition of DNA local distortion. Structure-based functional studies of eukaryotic Msh2-Msh6.

Bowers J, et al. (2001 Mar 9). MSH-MLH complexes formed at a DNA mismatch are disrupted by the PCNA sliding clamp.

Ellison AR, et al. (2001 Sep 1). Functional analysis of human MLH1 and MSH2 missense variants and hybrid human-yeast MLH1 proteins in Saccharomyces cerevisiae.

Drotschmann K, et al. (2002 Sep 4). Evidence for sequential action of two ATPase active sites in yeast Msh2-Msh6.

Kijas AW, et al. (2003 Aug 1). Msh2 separation of function mutations confer defects in the initiation steps of mismatch repair.

Lau PJ, et al. (2003 Jan 3). Transfer of the MSH2.MSH6 complex from proliferating cell nuclear antigen to mispaired bases in DNA.

Antony E, et al. (2003 Jul 1). Mismatch recognition-coupled stabilization of Msh2-Msh6 in an ATP-bound state at the initiation of DNA repair.

Clark AB, et al. (2004 Dec 24). Cadmium inhibits the functions of eukaryotic MutS complexes.

Banerjee S, et al. (2005). Cadmium inhibits mismatch repair by blocking the ATPase activity of the MSH2-MSH6 complex.

Jiang J, et al. (2005 Dec 9). Detection of high-affinity and sliding clamp modes for MSH2-MSH6 by single-molecule unzipping force analysis.

Mendillo ML, et al. (2005 Jun 10). Analysis of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 and MLH1-PMS1 complexes with DNA using a reversible DNA end-blocking system.

Mazur DJ, et al. (2006 Apr 7). Inhibition of Msh6 ATPase activity by mispaired DNA induces a Msh2(ATP)-Msh6(ATP) state capable of hydrolysis-independent movement along DNA.

Antony E, et al. (2006 Feb 3). Contribution of Msh2 and Msh6 subunits to the asymmetric ATPase and DNA mismatch binding activities of Saccharomyces cerevisiae Msh2-Msh6 mismatch repair protein.

Stone JE, et al. (2006 Jul). Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae.

Surtees JA, et al. (2006 Jul 14). Mismatch repair factor MSH2-MSH3 binds and alters the conformation of branched DNA structures predicted to form during genetic recombination.

Lee SD, et al. (2007 Feb 9). Saccharomyces cerevisiae MSH2-MSH3 and MSH2-MSH6 complexes display distinct requirements for DNA binding domain I in mismatch recognition.

Harrington JM, et al. (2007 Sep). Saccharomyces cerevisiae Msh2-Msh3 acts in repair of base-base mispairs.

Li F, et al. (2008 May 9). Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 8 genes with posterior transmembrane prediction > 50%

FOG03920
EOG8N5TBS

sce:MSH6

Genes: 32

SGD Description
Protein required for mismatch repair in mitosis and meiosis; forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p


PomBase Description
MutS protein homolog


AspGD Description
Ortholog(s) have role in maintenance of DNA repeat elements, mismatch repair and cytosol, nucleus localization


References

Iaccarino I, et al. (1996 Apr 1). MSH6, a Saccharomyces cerevisiae protein that binds to mismatches as a heterodimer with MSH2.

Marsischky GT, et al. (1996 Feb 15). Redundancy of Saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair.

Alani E, et al. (1996 Oct). The Saccharomyces cerevisiae Msh2 and Msh6 proteins form a complex that specifically binds to duplex oligonucleotides containing mismatched DNA base pairs.

Sia EA, et al. (1997 May). Microsatellite instability in yeast: dependence on repeat unit size and DNA mismatch repair genes.

Habraken Y, et al. (1998 Apr 17). ATP-dependent assembly of a ternary complex consisting of a DNA mismatch and the yeast MSH2-MSH6 and MLH1-PMS1 protein complexes.

Studamire B, et al. (1998 Dec). Saccharomyces cerevisiae Msh2p and Msh6p ATPase activities are both required during mismatch repair.

Flores-Rozas H, et al. (1998 Oct 13). The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations.

Bowers J, et al. (1999 Jun 4). A mutation in the MSH6 subunit of the Saccharomyces cerevisiae MSH2-MSH6 complex disrupts mismatch recognition.

Marsischky GT, et al. (1999 Mar 12). 'Saccharomyces cerevisiae MSH2/6 complex interacts with Holliday junctions and facilitates their cleavage by phage resolution enzymes.

Kolodner RD, et al. (1999 Oct 15). Germ-line msh6 mutations in colorectal cancer families.

Ni TT, et al. (1999 Sep). MSH2 and MSH6 are required for removal of adenine misincorporated opposite 8-oxo-guanine in S. cerevisiae.

Marsischky GT, et al. (1999 Sep 17). Biochemical characterization of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 complex and mispaired bases in DNA.

Das Gupta R, et al. (2000 Jan). Novel dominant mutations in Saccharomyces cerevisiae MSH6.

Flores-Rozas H, et al. (2000 Nov). Proliferating cell nuclear antigen and Msh2p-Msh6p interact to form an active mispair recognition complex.

Clark AB, et al. (2000 Nov 24). Functional interaction of proliferating cell nuclear antigen with MSH2-MSH6 and MSH2-MSH3 complexes.

Bowers J, et al. (2000 Sep 15). Analysis of yeast MSH2-MSH6 suggests that the initiation of mismatch repair can be separated into discrete steps.

Drotschmann K, et al. (2001 Dec 7). Asymmetric recognition of DNA local distortion. Structure-based functional studies of eukaryotic Msh2-Msh6.

Bowers J, et al. (2001 Mar 9). MSH-MLH complexes formed at a DNA mismatch are disrupted by the PCNA sliding clamp.

Mansour AA, et al. (2001 May). Control of GT repeat stability in Schizosaccharomyces pombe by mismatch repair factors.

Tornier C, et al. (2001 May). Requirement for Msh6, but not for Swi4 (Msh3), in Msh2-dependent repair of base-base mismatches and mononucleotide loops in Schizosaccharomyces pombe.

Hess MT, et al. (2002 Jul 12). Dominant Saccharomyces cerevisiae msh6 mutations cause increased mispair binding and decreased dissociation from mispairs by Msh2-Msh6 in the presence of ATP.

Drotschmann K, et al. (2002 Sep 4). Evidence for sequential action of two ATPase active sites in yeast Msh2-Msh6.

Lau PJ, et al. (2003 Jan 3). Transfer of the MSH2.MSH6 complex from proliferating cell nuclear antigen to mispaired bases in DNA.

Antony E, et al. (2003 Jul 1). Mismatch recognition-coupled stabilization of Msh2-Msh6 in an ATP-bound state at the initiation of DNA repair.

Clark AB, et al. (2004 Dec 24). Cadmium inhibits the functions of eukaryotic MutS complexes.

Banerjee S, et al. (2005). Cadmium inhibits mismatch repair by blocking the ATPase activity of the MSH2-MSH6 complex.

Jiang J, et al. (2005 Dec 9). Detection of high-affinity and sliding clamp modes for MSH2-MSH6 by single-molecule unzipping force analysis.

Mendillo ML, et al. (2005 Jun 10). Analysis of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 and MLH1-PMS1 complexes with DNA using a reversible DNA end-blocking system.

Mazur DJ, et al. (2006 Apr 7). Inhibition of Msh6 ATPase activity by mispaired DNA induces a Msh2(ATP)-Msh6(ATP) state capable of hydrolysis-independent movement along DNA.

Antony E, et al. (2006 Feb 3). Contribution of Msh2 and Msh6 subunits to the asymmetric ATPase and DNA mismatch binding activities of Saccharomyces cerevisiae Msh2-Msh6 mismatch repair protein.

Hess MT, et al. (2006 Jan 17). Biochemical basis for dominant mutations in the Saccharomyces cerevisiae MSH6 gene.

Stone JE, et al. (2006 Jul). Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae.

Clark AB, et al. (2007). Multiple functions for the N-terminal region of Msh6.

Chi A, et al. (2007 Feb 13). Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.

Lee SD, et al. (2007 Feb 9). Saccharomyces cerevisiae MSH2-MSH3 and MSH2-MSH6 complexes display distinct requirements for DNA binding domain I in mismatch recognition.

Shell SS, et al. (2007 Jun 26). Chimeric Saccharomyces cerevisiae Msh6 protein with an Msh3 mispair-binding domain combines properties of both proteins.

Holmes SF, et al. (2007 Mar 1). Specialized mismatch repair function of Glu339 in the Phe-X-Glu motif of yeast Msh6.

Wilson-Grady JT, et al. (2008 Mar). Phosphoproteome analysis of fission yeast.

Dixon SJ, et al. (2008 Oct 28). Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.

Pancaldi V, et al. (2012 Apr). Predicting the fission yeast protein interaction network.

Ryuko S, et al. (2012 Aug). Genome-wide screen reveals novel mechanisms for regulating cobalt uptake and detoxification in fission yeast.

Zamir L, et al. (2012 Feb 14). Tight coevolution of proliferating cell nuclear antigen (PCNA)-partner interaction networks in fungi leads to interspecies network incompatibility.

Chen Z, et al. (2012 Oct). A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.

Saito Y, et al. (2013). The proteasome factor Bag101 binds to Rad22 and suppresses homologous recombination.

Anver S, et al. (2014 Aug). Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Sideri T, et al. (2014 Dec 1). Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.

Swaffer MP, et al. (2016 Dec 15). CDK Substrate Phosphorylation and Ordering the Cell Cycle.

McDonald KR, et al. (2016 Sep). Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.

Lee J, et al. (2017 Feb 20). Chromatin remodeller Fun30^Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 2 genes with posterior transmembrane prediction > 50%

FOG03921
EOG8N5TBS

sce:MSH1

Genes: 32

SGD Description
DNA-binding protein of the mitochondria; involved in repair of mitochondrial DNA; has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis


PomBase Description
mitochondrial MutS protein Msh1 (predicted)


AspGD Description
Ortholog(s) have mitochondrion localization


References

Reenan RA, et al. (1992 Dec). Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functions.

Reenan RA, et al. (1992 Dec). Isolation and characterization of two Saccharomyces cerevisiae genes encoding homologs of the bacterial HexA and MutS mismatch repair proteins.

Chi NW, et al. (1994 Nov 25). Purification and characterization of MSH1, a yeast mitochondrial protein that binds to DNA mismatches.

Chi NW, et al. (1994 Nov 25). The effect of DNA mismatches on the ATPase activity of MSH1, a protein in yeast mitochondria that recognizes DNA mismatches.

Vanderstraeten S, et al. (1998 Sep 11). The role of 3'-5' exonucleolytic proofreading and mismatch repair in yeast mitochondrial DNA error avoidance.

Koprowski P, et al. (2002 Feb). A dominant mitochondrial mutator phenotype of Saccharomyces cerevisiae conferred by msh1 alleles altered in the sequence encoding the ATP-binding domain.

Sickmann A, et al. (2003 Nov 11). The proteome of Saccharomyces cerevisiae mitochondria.

Dzierzbicki P, et al. (2004 Apr 1). Repair of oxidative damage in mitochondrial DNA of Saccharomyces cerevisiae: involvement of the MSH1-dependent pathway.

Mookerjee SA, et al. (2005 Feb). Analysis of the functional domains of the mismatch repair homologue Msh1p and its role in mitochondrial genome maintenance.

Mookerjee SA, et al. (2006 Mar 20). Overlapping contributions of Msh1p and putative recombination proteins Cce1p, Din7p, and Mhr1p in large-scale recombination and genome sorting events in the mitochondrial genome of Saccharomyces cerevisiae.

Choi ES, et al. (2012 Sep). Factors that promote H3 chromatin integrity during transcription prevent promiscuous deposition of CENP-A(Cnp1) in fission yeast.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

McDonald KR, et al. (2016 Sep). Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 1 genes with posterior transmembrane prediction > 50%