FOG00381
EOG88KPSK
MAE0
sce:absent

Genes: 4

Protein description
NAD(P)-cytoplasmic malic enzyme


Features
[c];NAD(P)


AspGD Description
Protein similar to mitochondrial malic enzyme (NADP-specific); expression repressed by tunicamycin and DTT


Suggested Analysis
Investigate if ancestral MAE was [c] or [m]

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00382
EOG88KPSK

sce:absent

Genes: 1

Protein description
NADP-mitochondrial malic enzyme


Features
[m];NADP


Parent
ortholog:FOG00381


Suggested Analysis
Investigate if gene should be swapped with FOG00387


References

Zink MW, et al. (1967 Sep). Regulation of the "malic" enzyme in Neurospora crassa.

Zink MW, et al. (1972 May). Regulation of the two "malic" enzymes in Neurospora crassa.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00383


sce:absent

Genes: 1

Protein description
NADP-mitochondrial malic enzyme. (assumed from Rhodosporidium toruloides)


Features
[m]


Parent
ortholog:FOG00381


Suggested Analysis
Investigate if ancestral MAE was [c] or [m]

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 1 genes with posterior transmembrane prediction > 50%

FOG00384
EOG88KPSK

sce:absent

Genes: 2

Protein description
Cytoplasmic malic enzyme; important for high glucose concentration and anaerobic conditions


Features
[c];NAD


PomBase Description
malic enzyme, malate dehydrogenase (oxaloacetate decarboxylating), Mae2


References

Viljoen M, et al. (1998 Oct 15). Mutation of Gly-444 inactivates the S. pombe malic enzyme.

Viljoen M, et al. (1999 Apr 9). Transcriptional regulation of the Schizosaccharomyces pombe malic enzyme gene, mae2.

Groenewald M, et al. (2001 Jun). Factors involved in the regulation of the Schizosaccharomyces pombe malic enzyme gene.

Rodríguez-Gabriel MA, et al. (2006 Sep). Upf1, an RNA helicase required for nonsense-mediated mRNA decay, modulates the transcriptional response to oxidative stress in fission yeast.

Wilson-Grady JT, et al. (2008 Mar). Phosphoproteome analysis of fission yeast.

Roberts-Galbraith RH, et al. (2009 Jan 12). The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring.

Cho SJ, et al. (2010 Jun). Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis.

Singh NS, et al. (2011 Dec 6). SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.

Pancaldi V, et al. (2012 Apr). Predicting the fission yeast protein interaction network.

Zhou X, et al. (2013). A genome-wide screening of potential target genes to enhance the antifungal activity of micafungin in Schizosaccharomyces pombe.

Chen JS, et al. (2013 May). Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.

Carpy A, et al. (2014 Aug). Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Guo Y, et al. (2014 Jul). Large scale screening of genetic interaction with sgf73(+) in fission yeast.

Doi A, et al. (2015 Apr). Chemical genomics approach to identify genes associated with sensitivity to rapamycin in the fission yeast Schizosaccharomyces pombe.

Beckley JR, et al. (2015 Dec). A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Lee J, et al. (2017 Feb 20). Chromatin remodeller Fun30^Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 1 genes with posterior transmembrane prediction > 50%

FOG00385
EOG88KPSK

sce:absent

Genes: 1

Protein description
NAD-dependent malic enzyme


Parent
paralog:FOG00384


PomBase Description
NAD-dependent malic enzyme (predicted), partial

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00386
EOG88KPSK

sce:absent

Genes: 2

Protein description
NADP-mitochondrial malic enzyme, possible [c] localization; Malic enzyme has been isolated from Neurospora crassa which can bring about the reversible carboxylation of pyruvic acid. The enzyme is specific to L-malate and NADP and is activated by Mn++ and Mg++. The partially purified enzyme does not decarboxylate oxaloacetate but is competitively inhibited by it. This enzyme is synthesized only during the early stages of the growth cycle and is repressed by acetate.;;ang, tre are [m]; nadp only. Tre verified; different isoforms possible.


Features
[m]


Parent
ortholog:FOG00381


AspGD Description
Malate dehydrogenase (NADP-specific)


References

Zink MW, et al. (1967 Sep). Regulation of the "malic" enzyme in Neurospora crassa.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00387
EOG88KPSK

sce:absent

Genes: 1

Protein description
NAD(P?)-cytoplasmic malic enzyme. Enzyme does not share localization with FOG00386 despite being monophyletic. "Malic enzyme has been isolated from Neurospora crassa which can bring about the reversible carboxylation of pyruvic acid. The enzyme is specific to L-malate and NADP and is activated by Mn++ and Mg++. The partially purified enzyme does not decarboxylate oxaloacetate but is competitively inhibited by it. This enzyme is synthesized only during the early stages of the growth cycle and is repressed by acetate"


Features
[c]


Parent
ortholog:FOG00381


References

Zink MW, et al. (1967 Sep). Regulation of the "malic" enzyme in Neurospora crassa.

Zink MW, et al. (1972 May). Regulation of the two "malic" enzymes in Neurospora crassa.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00388


sce:absent

Genes: 10

Protein description
Cytoplasmic malic enzyme, ancestral budding yeast, NAD preferred but can also use NADP to lesser extent. Evidence of cofactor usage in yli and lst


Features
[c]

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00389
EOG88KPSK

sce:absent

Genes: 1

Protein description
mitochondrial malic enzyme, YLI paralog, NAD and NADP cofactor


Features
[m];NAD(P)


Parent
ortholog:FOG00388


References

Zhang H, et al. (2013 Dec). Regulatory properties of malic enzyme in the oleaginous yeast, Yarrowia lipolytica, and its non-involvement in lipid accumulation.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00390
EOG88KPSK

sce:absent

Genes: 14

Protein description
mitochondrial malic enzyme assume NAD and NADP cofactor. C. tropicalis paper shows higher activity with NAD. Strong evidence of [m]


Features
[m];

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00391


sce:absent

Genes: 1

Protein description
MAE [c], HVA paralog, assume dual cofactor; unambigious based on fna sequence analysis


Features
[c];NAD(P)?


Parent
ortholog?:FOG00390

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00392
EOG88KPSK

sce:MAE1

Genes: 2

Protein description
NADP-mitochondrial malic enzyme


Features
[m];NADP


Parent
ortholog:FOG00390


SGD Description
Mitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids


References

Boles E, et al. (1998 Jun). Identification and characterization of MAE1, the Saccharomyces cerevisiae structural gene encoding mitochondrial malic enzyme.

Sickmann A, et al. (2003 Nov 11). The proteome of Saccharomyces cerevisiae mitochondria.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%

FOG00393
EOG88KPSK

sce:absent

Genes: 1

Protein description
NADP?-mitochondrial malic enzyme, vpo ohnolog, unknown cofactor but assume NADP


Features
[m];NADP?


Parent
ohnolog:FOG00390

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 0 genes with posterior transmembrane prediction > 50%