FOG00277
EOG8CZ8WX
PFK2
sce:absent

Genes: 9

Protein description
Homo-oligomeric phosphofructokinase


Features
homo-oligomeric


PomBase Description
6-phosphofructokinase pfk1


AspGD Description
6-phosphofructo-1-kinase; posttranslationally processed; the native enzyme is first cleaved to an inactive fragment that regains its activity after phosphorylation


Suggested Analysis
Find differences in allosteric regulation in less characterized yeasts (nfu, aru)


References

Habison A, et al. (1983 Mar 1). Partial purification and regulatory properties of phosphofructokinase from Aspergillus niger.

Reuter R, et al. (2000 Oct). Purification, molecular and kinetic characterization of phosphofructokinase-1 from the yeast Schizosaccharomyces pombe: evidence for an unusual subunit composition.

Muthuvijayan V, et al. (2004). In silico reconstruction of nutrient-sensing signal transduction pathways in Aspergillus nidulans.

Sims AH, et al. (2004 Feb). Use of expressed sequence tag analysis and cDNA microarrays of the filamentous fungus Aspergillus nidulans.

Flores CL, et al. (2005 May). The dimorphic yeast Yarrowia lipolytica possesses an atypical phosphofructokinase: characterization of the enzyme and its encoding gene.

Flipphi M, et al. (2009 Mar). Biodiversity and evolution of primary carbon metabolism in Aspergillus nidulans and other Aspergillus spp.

Masuo S, et al. (2010 Dec). Global gene expression analysis of Aspergillus nidulans reveals metabolic shift and transcription suppression under hypoxia.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 2 genes with posterior transmembrane prediction > 50%

FOG00278
EOG8CZ8WX
PFK2.2
sce:PFK2

Genes: 25

Protein description
Heter-oligomeric phosphofructokinase beta subunit


Features
heter-oligomeric subunit


Parent
ortholog:FOG00277


SGD Description
Beta subunit of heterooctameric phosphofructokinase; involved in glycolysis; indispensable for anaerobic growth; activated by fructose-2,6-bisphosphate and AMP; mutation inhibits glucose induction of cell cycle-related genes


Suggested Analysis
Find differences in allosteric regulation in less characterized yeasts (nfu, aru)


References

Kopperschläger G, et al. (1977 Dec 1). Physicochemical parameters and subunit composition of yeast phosphofructokinase.

Bañuelos M, et al. (1977 Sep 25). Activation by phosphate of yeast phosphofructokinase.

Navon G, et al. (1979 Oct 16). Phosphorus-31 nuclear magnetic resonance studies of wild-type and glycolytic pathway mutants of Saccharomyces cerevisiae.

Avigad G, et al. (1981 Oct 15). Stimulation of yeast phosphofructokinase activity by fructose 2,6-bisphosphate.

Clifton D, et al. (1982 Apr 13). Mutant studies of yeast phosphofructokinase.

Lobo Z, et al. (1983 Feb 10). Phosphofructokinase mutants of yeast. Biochemistry and genetics.

Nissler K, et al. (1983 Feb 28). Similarity of activation of yeast phosphofructokinase by AMP and fructose-2,6-bisphosphate.

Hofmann E, et al. (1985). Temporal organization of the phosphofructokinase/fructose-1,6-biphosphatase cycle.

Nissler K, et al. (1985). An electron microscopy study of the quarternary structure of yeast phosphofructokinase.

Heinisch J, et al. (1986 Jan). Isolation and characterization of the two structural genes coding for phosphofructokinase in yeast.

Heinisch J, et al. (1989 May 30). The phosphofructokinase genes of yeast evolved from two duplication events.

Heinisch J, et al. (1993 May). Molecular genetics of phosphofructokinase in the yeast Kluyveromyces lactis.

Kopperschläger G, et al. (1993 Oct 15). Limited proteolysis of yeast phosphofructokinase. Sequence locations of cleavage sites created by the actions of different proteinases.

Arvanitidis A, et al. (1994 Mar 25). Studies on the function of yeast phosphofructokinase subunits by in vitro mutagenesis.

Heinisch JJ, et al. (1996 Jul 5). A yeast phosphofructokinase insensitive to the allosteric activator fructose 2,6-bisphosphate. Glycolysis/metabolic regulation/allosteric control.

Lorberg A, et al. (1999 Feb). Genetic and biochemical characterization of phosphofructokinase from the opportunistic pathogenic yeast Candida albicans.

Rodicio R, et al. (2000 Dec 29). Single point mutations in either gene encoding the subunits of the heterooctameric yeast phosphofructokinase abolish allosteric inhibition by ATP.

Reuter R, et al. (2000 Oct). Purification, molecular and kinetic characterization of phosphofructokinase-1 from the yeast Schizosaccharomyces pombe: evidence for an unusual subunit composition.

Gruhler A, et al. (2005 Mar). Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.

Brandina I, et al. (2006 Sep-Oct). Enolase takes part in a macromolecular complex associated to mitochondria in yeast.

Chi A, et al. (2007 Feb 13). Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.

Barnard E, et al. (2008 May). Detection and localisation of protein-protein interactions in Saccharomyces cerevisiae using a split-GFP method.

Banaszak K, et al. (2011 Mar 25). The crystal structures of eukaryotic phosphofructokinases from baker's yeast and rabbit skeletal muscle.

Chan CY, et al. (2014 Jul 11). Yeast phosphofructokinase-1 subunit Pfk2p is necessary for pH homeostasis and glucose-dependent vacuolar ATPase reassembly.

Gustavsson AK, et al. (2014 Jun). Allosteric regulation of phosphofructokinase controls the emergence of glycolytic oscillations in isolated yeast cells.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 5 genes with posterior transmembrane prediction > 50%

FOG00279
EOG8CZ8WX
PFK1
sce:PFK1

Genes: 24

Protein description
Heter-oligomeric phosphofructokinase alpha subunit


Features
heter-oligomeric subunit


Parent
paralog:FOG00278


SGD Description
Alpha subunit of heterooctameric phosphofructokinase; involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes


Suggested Analysis
Find differences in allosteric regulation in less characterized yeasts (nfu, aru)


References

Kopperschläger G, et al. (1977 Dec 1). Physicochemical parameters and subunit composition of yeast phosphofructokinase.

Bañuelos M, et al. (1977 Sep 25). Activation by phosphate of yeast phosphofructokinase.

Clifton D, et al. (1978 Jan). Glycolysis mutants in Saccharomyces cerevisiae.

Avigad G, et al. (1981 Oct 15). Stimulation of yeast phosphofructokinase activity by fructose 2,6-bisphosphate.

Clifton D, et al. (1982 Apr 13). Mutant studies of yeast phosphofructokinase.

Nissler K, et al. (1983 Feb 28). Similarity of activation of yeast phosphofructokinase by AMP and fructose-2,6-bisphosphate.

Hofmann E, et al. (1985). Temporal organization of the phosphofructokinase/fructose-1,6-biphosphatase cycle.

Nissler K, et al. (1985). An electron microscopy study of the quarternary structure of yeast phosphofructokinase.

Heinisch J, et al. (1986 Jan). Isolation and characterization of the two structural genes coding for phosphofructokinase in yeast.

Heinisch J, et al. (1989 May 30). The phosphofructokinase genes of yeast evolved from two duplication events.

Heinisch J, et al. (1993 May). Molecular genetics of phosphofructokinase in the yeast Kluyveromyces lactis.

Kopperschläger G, et al. (1993 Oct 15). Limited proteolysis of yeast phosphofructokinase. Sequence locations of cleavage sites created by the actions of different proteinases.

Arvanitidis A, et al. (1994 Mar 25). Studies on the function of yeast phosphofructokinase subunits by in vitro mutagenesis.

Heinisch JJ, et al. (1996 Jul 5). A yeast phosphofructokinase insensitive to the allosteric activator fructose 2,6-bisphosphate. Glycolysis/metabolic regulation/allosteric control.

Bär J, et al. (1997 Nov). Purification and characterization of phosphofructokinase from the yeast Kluyveromyces lactis.

Lorberg A, et al. (1999 Feb). Genetic and biochemical characterization of phosphofructokinase from the opportunistic pathogenic yeast Candida albicans.

Rodicio R, et al. (2000 Dec 29). Single point mutations in either gene encoding the subunits of the heterooctameric yeast phosphofructokinase abolish allosteric inhibition by ATP.

Gruhler A, et al. (2005 Mar). Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.

Flores CL, et al. (2005 May). The dimorphic yeast Yarrowia lipolytica possesses an atypical phosphofructokinase: characterization of the enzyme and its encoding gene.

Brandina I, et al. (2006 Sep-Oct). Enolase takes part in a macromolecular complex associated to mitochondria in yeast.

Benjamin S, et al. (2007 Sep). Structures of S. pombe phosphofructokinase in the F6P-bound and ATP-bound states.

Barnard E, et al. (2008 May). Detection and localisation of protein-protein interactions in Saccharomyces cerevisiae using a split-GFP method.

Banaszak K, et al. (2011 Mar 25). The crystal structures of eukaryotic phosphofructokinases from baker's yeast and rabbit skeletal muscle.

Starita LM, et al. (2012 Jan). Sites of ubiquitin attachment in Saccharomyces cerevisiae.

Van Damme P, et al. (2012 Jul 31). N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 5 genes with posterior transmembrane prediction > 50%

FOG00280
EOG8CZ8WX
PFK3
sce:absent

Genes: 1

Protein description
Uncharacterized phosphofructokinase, ohnolog

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 1 genes with posterior transmembrane prediction > 50%