FOG00028
EOG8T4B96
RPL19
sce:RPL19A;RPL19B

Genes: 36

Protein description
RPL19 Large ribosome protein subunit


SGD Description
Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication|Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication


PomBase Description
60S ribosomal protein L19


AspGD Description
Ortholog(s) have cell surface, cytosolic large ribosomal subunit, preribosome, large subunit precursor localization


References

Otaka E, et al. (1983). Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe.

Shannon KW, et al. (1988 Jun 5). Isolation and characterization of the Saccharomyces cerevisiae MIS1 gene encoding mitochondrial C1-tetrahydrofolate synthase.

Takakura H, et al. (1992 Mar 15). NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae.

Gish W, et al. (1993 Mar). Identification of protein coding regions by database similarity search.

Song JM, et al. (1995 Apr 15). Nucleotide sequence and characterization of the Saccharomyces cerevisiae RPL19A gene encoding a homolog of the mammalian ribosomal protein L19.

Song JM, et al. (1996 Sep). Organization and characterization of the two yeast ribosomal protein YL19 genes.

Planta RJ, et al. (1998 Mar 30). The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae.

Spahn CM, et al. (2001 Nov 2). Structure of the 80S ribosome from Saccharomyces cerevisiae--tRNA-ribosome and subunit-subunit interactions.

Lee SW, et al. (2002 Apr 30). Direct mass spectrometric analysis of intact proteins of the yeast large ribosomal subunit using capillary LC/FTICR.

Spahn CM, et al. (2004 Mar 10). Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation.

Ben-Shem A, et al. (2010 Nov 26). Crystal structure of the eukaryotic ribosome.

Ben-Shem A, et al. (2011 Dec 16). The structure of the eukaryotic ribosome at 3.0 Å resolution.

Starita LM, et al. (2012 Jan). Sites of ubiquitin attachment in Saccharomyces cerevisiae.

Ban N, et al. (2014 Feb). A new system for naming ribosomal proteins.

Murray J, et al. (2016 May 9). Structural characterization of ribosome recruitment and translocation by type IV IRES.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 1 genes with posterior transmembrane prediction > 50%

FOG00029
EOG8T4B96

sce:GCN1

Genes: 33

Protein description
Positive regulator of the Gcn2p kinase activity


SGD Description
Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA


PomBase Description
translation elongation regulator Gcn1 (predicted)


AspGD Description
Ortholog(s) have role in regulation of translational elongation and cytosolic ribosome, extracellular region, mitochondrion localization


Suggested Analysis
Likely a suprious homolog or an ancient duplication; Few conserved motifs in multiple sequence alignment; ortholog has insertions relative to apparent ancestral protein


References

Marton MJ, et al. (1993 Jun). GCN1, a translational activator of GCN4 in Saccharomyces cerevisiae, is required for phosphorylation of eukaryotic translation initiation factor 2 by protein kinase GCN2.

Vazquez de Aldana CR, et al. (1995 Jul 3). GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2 alpha kinase GCN2 in amino acid-starved cells.

Marton MJ, et al. (1997 Aug). Evidence that GCN1 and GCN20, translational regulators of GCN4, function on elongating ribosomes in activation of eIF2alpha kinase GCN2.

Garcia-Barrio M, et al. (2000 Apr 17). Association of GCN1-GCN20 regulatory complex with the N-terminus of eIF2alpha kinase GCN2 is required for GCN2 activation.

Sattlegger E, et al. (2000 Dec 1). Separate domains in GCN1 for binding protein kinase GCN2 and ribosomes are required for GCN2 activation in amino acid-starved cells.

Kubota H, et al. (2000 Jul 7). GI domain-mediated association of the eukaryotic initiation factor 2alpha kinase GCN2 with its activator GCN1 is required for general amino acid control in budding yeast.

Kubota H, et al. (2001 May 18). Budding yeast GCN1 binds the GI domain to activate the eIF2alpha kinase GCN2.

Sattlegger E, et al. (2004 Jul 16). YIH1 is an actin-binding protein that inhibits protein kinase GCN2 and impairs general amino acid control when overexpressed.

Sattlegger E, et al. (2005 Apr 22). Polyribosome binding by GCN1 is required for full activation of eukaryotic translation initiation factor 2{alpha} kinase GCN2 during amino acid starvation.

Wout PK, et al. (2009 Jul). Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on Polyribosomes with Gcn1.

Sattlegger E, et al. (2011 Mar 25). Gcn1 and actin binding to Yih1: implications for activation of the eIF2 kinase GCN2.

Visweswaraiah J, et al. (2011 Oct 21). Evidence that eukaryotic translation elongation factor 1A (eEF1A) binds the Gcn2 protein C terminus and inhibits Gcn2 activity.

Saykhedkar S, et al. (2012 Jul 26). A time course analysis of the extracellular proteome of Aspergillus nidulans growing on sorghum stover.

Waller T, et al. (2012 May). Evidence that Yih1 resides in a complex with ribosomes.

Visweswaraiah J, et al. (2012 Nov 2). Overexpression of eukaryotic translation elongation factor 3 impairs Gcn2 protein activation.

Cambiaghi TD, et al. (2014 Jan 10). Evolutionarily conserved IMPACT impairs various stress responses that require GCN1 for activating the eIF2 kinase GCN2.

Lee SJ, et al. (2015 Mar 15). Gcn1 contacts the small ribosomal protein Rps10, which is required for full activation of the protein kinase Gcn2.

Mitochondrial localization predictions
Predotar	TargetP	MitoProt
Raw data
Phobius transmembrane predictions 30 genes with posterior transmembrane prediction > 50%